摘要
以火焰树种子为外植体,研究不同培养基对种子萌发、茎段增殖和生根培养的影响。结果表明,火焰树种子经预处理后,用70%酒精消毒30 s,再经2%Na Cl O浸泡15 min的灭菌效果较好,污染率为16.7%;种子萌发最适培养基为MS+6-BA2.0 mg·L-1+GA3 0.1 mg·L-1,萌发率达89.1%;较合适的茎段继代增殖培养基为WPM+6-BA 1.5 mg·L-1+NAA 0.2 mg·L-1+活性炭(AC)0.5 g·L-1;最适的生根培养基为WPM+NAA 0.2 mg·L-1+IBA 0.2 mg·L-1+AC 1 g·L-1,生根率达96.7%,每株平均生根数为6.8条。
Using Spathodea campanulata seeds as explants, the effects of different culture medilams on seed germination, bud multiplication and rooting were studied. The results showed that the best sterilization method for S. campanulata seeds was to soak them into 70% alcohol for 30 s and 2% NaCIO for 15 min after pretreat- ment, with the contamination rate of 16.7%; the best medium for seed germination was MS＋6-BA 2.0 mg·L1＋ GA3 0.1 mg.L1, and the germination rate was up to 89.1%; the suitable medium for bud subculture and prolifer- ation was WPM＋6-BA 1.5 mg.L1＋NAA 0.2 mg·L-1＋activated carbon （AC） 0.5 g·L-1; the optimal rooting medi- um was WPM＋NAA 0.2 mg.LI＋IBA 0.2 mg.LI＋AC 1.0 g·L-1, on which the rooting rate reached 96.7% and the average number of roots was 6.8.
出处
《植物生理学报》
CAS
CSCD
北大核心
2015年第5期709-714,共6页
Plant Physiology Journal
基金
闽南师范大学园林植物生长发育与生态配置创新团队(139004)
关键词
火焰树
种子
组织培养
快速繁殖
Spathodea campanulata
seeds
tissue culture
rapid propagation
