摘要
为了解环洞庭湖区活禽批发市场和水禽场H6亚型禽流感病毒的分布和流行情况,分别于2009—2011年对环洞庭湖地区活禽批发市场、2011—2013年对水禽场的H6亚型禽流感进行了系统监测。对在活禽批发市场上采集的拭子样品先由尿囊腔接种SPF鸡胚,然后用血凝试验(HA)测定所收集的尿囊液,对HA测定有滴度的再进行血凝抑制试验(HI),并与RT–PCT方法相结合鉴定病毒的亚型。检测结果显示:在环洞庭湖区的5个活禽批发市场上都分离到H6亚型禽流感,并且在鸡、鸭、鹅拭子中都分离到该病毒,总的分离率为6.49%;水禽场H6亚型禽流感病毒群体阳性率达3.6%,拭子的H6亚型病毒分离率为0.29%,活禽批发市场鸭拭子H6亚型禽流感病毒分离率为9.58%,表明市场环节水禽H6亚型禽流感的隐性带毒率比养殖环节高,在一定程度上说明禽流感在活禽批发市场存在水平传播的风险。
In order to understand the distribution and prevent the prevalence of subtype avian influenza virus of H6, surveillance for H6 virus was implemented in the wholesale markets of live poultry and waterfowl fields around the Dongting Lake. Samples were firstly injected to embryos of SPF chicken by allantoic cavity, and then the allantoic fluid was collected and tested using hemagglutination（HA）. If a positive result was gotten, then subtypes of the virus would be determined by hemoagglutination inhibition（HI） and RT–PCT. The surveillance demonstrated that H6 virus was isolated from chickens, ducks and geese sampled at all wholesale markets of live poultry with a total separation rate of 6.49%. The positive rate of subtype virus of H6 in the waterfowl fields was 3.6%, and the separation rate from swabs was 0.29%, while, the separation rate from duck swabs was 9.58% in the wholesale markets of live poultry. These results indicated that the hidden risk of H6 virus in the wholesale markets of live poultry was higher than that of in waterfowl fields.
出处
《湖南农业大学学报（自然科学版）》
CAS
CSCD
北大核心
2015年第3期299-302,共4页
Journal of Hunan Agricultural University(Natural Sciences)
基金
国家"973"自然科学基金项目(2011CB505001)
关键词
活禽批发市场
水禽场
H6亚型禽流感病毒
洞庭湖区
The wholesale market of live poultry waterfowl field subtype avian influenza virus of H6 Dongting Lake area
