摘要
目的：探讨吗啡依赖大鼠觅药消退期边缘下区（infralimbic cortex, IL）遥测脑电活动的变化对觅药行为的影响及其机制。方法将SD大鼠随机分成模型组和对照组，在IL区实施脑立体定位电极埋植，模型组大鼠依次制成吗啡依赖和消退模型，应用无线遥测系统记录大鼠消退期IL区脑电活动的变化。结果模型组大鼠戒断1~2 d白箱停留时间明显长于吗啡注射前及对照组；消退训练后1~2 d，模型组大鼠白箱停留时间比戒断期明显缩短，与吗啡注射前及对照组相比，无明显差异。模型组与对照组比较，戒断2 d停留白箱时，IL区脑电β波显著增多，δ波显著减少；从黑箱向白箱穿梭时，δ波显著增多，α波和β波显著减少。消退2 d，模型组大鼠停留白箱时，IL区脑电比对照组θ波明显减少，与戒断期比较β波和θ波明显减少，δ波明显增多；从黑箱向白箱穿梭时，与对照组相比IL脑电无显著差异，但与戒断期比较，δ波明显减少，α波和β波明显增多。结论吗啡依赖大鼠觅药消退期IL区脑电活动发生异常变化，这种异常脑电的产生可能影响觅药动机的形成，进而抑制觅药行为的表达和维持。
Objective To investigate the changes of telemetry electrical activity in the infralimbic cortex （IL） of morphine-dependent rats with extinguished drug-seeking behavior. Methods SD rats were randomly divided into model group and control group and received operations of brain stereotaxic electrode embedding in the IL. The rats in the model group were induced to acquire morphine dependence and then received subsequent extinction training, and the changes of electrical activity in the IL were recorded with a physical wireless telemetry system. Results In rats with morphine dependence, the time staying in the white box was significantly longer on days 1 and 2 after withdrawal than that before morphine injection and that of the control rats, but was obviously reduced on days 1 and 2 after extinction training to the control level. Compared with the control group, the morphine-dependent rats on day 2 following withdrawal showed significantly increased β wave and decreased δ wave when they stayed in the white box but significantly increased δ wave and decreased α wave and β wave when they shuttled from the black to the white box. On day 2 of extinction, the model rats, when staying in the white box, showed significantly decreasedθwave compared with that of the control rats group but decreasedβwave andθwave and increasedδwave compared with those in the withdrawal period. When they shuttled from black to white box, the model rats showed decreased δ wave and increased α wave and β wave compared with those in the withdrawal period. Conclusion Morphine-dependent rats have abnormal changes of electrical activity in the IL in drug-seeking extinction to affect their drug-seeking motive and inhibit the expression and maintenance of drug-seeking behaviors.
出处
《南方医科大学学报》
CAS
CSCD
北大核心
2015年第5期733-737,共5页
Journal of Southern Medical University
基金
安徽省自然科学基金(090413096
10040606Q66)
皖南医学院中青年科研基金(WK201406)
关键词
吗啡依赖
觅药消退
边缘下区
遥测
脑电活动
morphine dependence
drug-seeking extinction
infralimbic cortex
telemetry
brain electrical activity
