摘要
目的研究不同浓度生物活性玻璃（bioactive glass，BG）对3种浮游细菌及其形成的单菌种菌斑、三菌种早期龈下混合菌斑的抗菌效果。方法测定血链球菌（Streptococcus sanguinis，Ss）、具核梭杆菌（Fusobacterium nucleatum，Fn）、伴放线放线杆菌（Actinobacillus actinomycetemcomitans，Aa）的最小抑菌浓度（minimal inhibitory concentration，MIC）、最小杀菌浓度（minimal bactericidal concentration，MBC）、最小菌斑清除浓度（minimal biofilm eradication concentration，MBEC）。20、40、80mg／mL的生物活性玻璃和惰性玻璃二氧化硅（SiO2）处理24h早期三菌混合菌斑，于激光共聚焦显微镜（confocal laser scanning microscopy，CLSM）下摄片观察分析生物膜厚度和平均荧光强度。结果BG对菌斑的MBEC明显大于对浮游菌的MIC和MBC，MBEC为MIC的2-16倍。随着生物玻璃浓度加大，混合菌斑生物膜厚度变薄，团块减小，菌斑活性比减低，与空白对照组和阳性对照SiO2组有显著统计学差异（P〈0．05）。结论BG可抑制和杀灭血链球菌、聚合梭杆菌、伴放线放线杆菌浮游菌及其单菌种菌斑生物膜，对浮游细菌效果优于菌斑生物膜。低浓度BG对龈下混合菌斑有一定抗菌效果。
Objective To evaluate the antibacterial effects of bioactive glass （BG） of different concentrations on three species of planktonic bacteria,single bacterial biofilms formed by the planktonic bacteria,and the subgingival early mixed bacterial plaque. Meth- ods The minimal inhibitory concentration （MIC） , minimal bactericidal concentration（MBC） , minimal biofilm eradicationeoncentration （MBEC） of Streptococcus sanguinis （Ss） ,Actinobacillus actinomycetemcomitans （Aa） and Fusobacterium nucleatum （Fn） were assessed. Inert silica glass （SiO2 ）and bioactive glass of 20,40,80 mg/mL were used to treat early mixed bacterial plaque for 24 h. Confocal laser scanning microscopy （CLSM） was used to observe and analyze the thickness and average fluorescence intensity of the plaque. Results The MBECs of BG to single bacterial plaque biofilm were significantly greater than the MICs and MBCs of planktonic bacteria. MBECs were 2 - 16 times greater than MICs. With the concentrations of bioglass increasing,there was a relative decrease in the thickness,mass and vitality of the mixed plaque. Statistically there was a significant difference between the blank control group and SiO2 groups （ P 〈 0.05）. Conclusions BG can inhibit and kill planktonic forms of Streptococcus sanguinis, Actinobacillus actinomycetemcomitans and Fusobacterium nucleatum as well as the single form of bacterial plaque biofilm. However,the effect on single form of bacterial plaque biofilm was much better. BG of low concentration had certain antibacterial effects on the subgingival early mixed bacterial plaque.
出处
《口腔医学》
CAS
2015年第5期354-358,共5页
Stomatology
基金
江苏高校优势学科建设工程资助项目(2014-37)
关键词
生物活性玻璃
龈下菌斑
生物膜
抗菌效果
bioactive glass
subgingival plaque
biofilm
antibacterial effect
