摘要
目的通过检测组织激肽释放酶7（KLK7）在不同级别宫颈病变组织中的表达，探讨KLK7在宫颈癌前病变进展中的作用，筛选高危个体，指导临床上对CIN 的个体化治疗。方法收集深圳市人民医院妇科2012至2014年手术切除标本120例，通过免疫组化方法检测CIN 组织中KLK7的表达，并以慢性宫颈炎组织，宫颈鳞癌作对照，分析宫颈癌前病变组织中 KLK7的表达水平，对其表达水平与CIN 不同级别的相关性进行分析。结果本研究证实KLK7广泛存在于CIN 的细胞浆和细胞核中，慢性宫颈炎组和CIN 组及宫颈癌组间差异有统计学意义，CIN 分级后有显著性差异。高级别CIN 及宫颈癌中KLK7阳性率显著高于低级别CIN 及慢性宫颈炎。慢性宫颈炎组织中KLK7有表达，但阳性结果中以低表达为主，阳性率52.5%。且随着CIN 级别升高，阳性细胞数显著增多，染色逐渐加深，阳性率从CIN1的77.5%增加到CIN3的97.5%。结论 KLK7在 CIN 的发生、发展连续过程中的表达逐渐增强，可能参与宫颈癌前病变的发生发展，KLK7高表达可能与宫颈癌发生相关。KLK7检测可能预测宫颈癌前病变预后。
Objective Through the detection of kallikrein 7 （KLK7） expression in different grades of cervical lesion tissues,to explore the role of KLK7 in the development of cervical precancerous lesion,screening of high-risk individuals,individual guide the clinical treatment for CIN. Methods Paraffin sections of 120 cases were collected,the expression of KLK7 by immunohistochemical method in the CIN tissue,and the tissue of chronic cervicitis,cervical squamous cell carcinoma as control,to analyze the expression level of KLK7 in tissues of cervical precancerous lesions,the correlation of their expression level and CIN at different levels of analysis. Results This study showed that KLK7 widely exists in the CIN cytoplasm and nucleus in chronic cervicitis,there was significant difference between the group and the CIN group and cervical carcinoma group,there was significant difference in CIN classification.The positive rate of KLK7 in high level CIN and cervical cancer were significantly higher than those of low grade CIN and chronic cervicitis.Chronic cervicitis tissue KLK7 expression,but positive results in low expression, the positive rate was 52.5%,but weakly positive expression of the total positive rate of 92%.And with the increase of CIN level, the number of positive cells increased significantly,staining gradually deepened,the positive rate of CIN1 increased from 77.5% to 97.5% of CIN3,the strong positive rate to 72.5% of CIN3 rise from 5% of CIN1.The positive rate of cervical cancer in moderate amounts to 87.5%,while the CIN1 and CIN2 strong positive rate was only 21.2%. （P〈0.05）. Conclusion The expression of KLK7 in the development of CIN,the continuous process of gradually increased,may be involved in the tumorigenesis of cervical precancerous lesion,the high expression of KLK7 may be associated with cervical cancer.Detection of KLK7 may predict the prognosis of cervical precancerous lesions.
出处
《中国临床实用医学》
2015年第2期36-39,共4页
China Clinical Practical Medicine
基金
深圳市科技计划项目（201302018）
关键词
组织激肽释放酶7
宫颈上皮内瘤样病变
免疫组织化学
Kallikerein 7 （ KLK7 ）
Cervical intraepithelial neoplasia （ CIN ）
Immunohistochemistry
