摘要
甲羟戊酸 （MVA）途径是萜类化合物生物合成的重要途径,该研究在厚朴转录组测序基础上,利用生物信息学方法对MVA途径中相关基因MoACOT,MoHMGS,MoHMGR,MoMK进行全面的生物信息学分析。结果表明,MoACOT和MoMK为稳定的亲水蛋白,而MoHMGS,MoHMGR为不稳定的亲水蛋白;二级结构均为混合型结构的蛋白质,α-螺旋是所有基因多肽链中大量的结构元件;利用同源建模法对三级空间结构进行了预测;跨膜域分析显示MoACOT,MoHMGS,MoMK无跨膜区, MoHMGR具有2个跨膜区,分别为39-61 aa和82-104 aa;系统进化树分析表明MoACOT,MoHMGS,MoHMGR,MoMK均与被子植物或双子叶植物遗传距离较近,符合遗传进化规律;基因表达水平显示MoACOT,MoHMGS,MoHMGR,MoMK表达水平在厚朴与凹叶厚朴间均无显著差异。研究结果为解析厚朴萜类生物合成MVA途径的分子机制提供理论依据。
Methyl valerate （MVA） pathway is one of the important ways for synthesis of terpenoids. This study was based on data of the transcriptome sequencing of Magnolia officinalis,the associated genes MoACOT, MoHMGS, MoHMGR, MoMK in methyl valerate （MVA） pathway,were completed in detail by using bioinformatics methods. The results of analysis showed that MoACOT and MoMK were stable hydrophobic proteins,MoHMGS and MoHMGR were unstable hydrophobic protein. The secondary structures of all proteins were hybrid architecture,and alpha helical were the major motifs. There were no clear transmembrane domains in MoACOT,MoHMGS and MoMK,but two transmembrane domains were founded in MoHMGR which were from 39-61 aa and 82-104 aa resepectively. The results of evolutionary relationship analysis showed that MoACOT,MoHMGS,MoHMGR and MoMK had relative close relationship to angiosperm or dicotyledonous plants,and accorded with genetic evolution rule. From transcriptome data,transcripted level of MoACOT,MoHMGS,MoHMGR,MoMK in M. officinalis and M. officinalis var. biloba was not significantly different. The result provided theoretical reference for study on Methyl valerate （MVA） pathway of terpenoid of M. officinalis.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2015年第11期2077-2083,共7页
China Journal of Chinese Materia Medica
基金
国家杰出青年科学基金项目(81325023)
国家高技术研究发展计划(863)项目(SS2014AA022201)
关键词
厚朴
MVA途径
萜类
转录组
生物信息学
Magnolia officinaliz
MVA pathway
terpenoids
transcriptome
bioinformatics
