摘要
目的研究4种丹参酮类化合物对拘束应激引起小鼠肝损伤的保护作用。方法采用拘束负荷法造成小鼠应激性肝损伤。应用赖氏法测定小鼠血浆中ALT活性,TBARS法测定血浆及肝组织匀浆中的MDA含量,抗氧化能力指数(ORAC)法测定血浆抗氧化能力指数,HPLC法测定血浆及肝组织匀浆中维生素C和GSH水平,以及ORAC法测定和观察丹参酮类化合物的体内和体外抗氧化活性。结果与拘束模型组相比,4种丹参酮类化合物均可明显降低应激小鼠血浆ALT水平,提高肝组织匀浆的抗氧化能力指数、维生素C和GSH水平,并降低MDA含量。4种丹参酮类化合物体外也显示出较强的抗氧化能力。在体内和体外实验中,二氢丹参酮的抗氧化作用均明显优于其他3种丹参酮类化合物。结论丹参酮类化合物对拘束应激引起的小鼠肝损伤具有一定的保护作用,其作用机制可能部分来自于其抗氧化活性。
Aim To observe the protective effects of tanshinones （tanshinone IIA, tanshinone I, cryptotanshinone and dihydrotanshinone） against liver injury in mice loaded with restraint stress. Methods The liver injury model was established under 12 h restraint stress in mice 5 days after tanshinones treatment. The hepatoprotective effects were evaluated by assessing alanine aminotransferase （ALT） levels in plasma. The contents of vitamin C, GSH and malondialdehyde （MDA） in liver were performed by HPLC and TBARS methods, respectively. Oxygen radical absorbance capacity （ORAC） assay was used to measure the antioxidant capacity. Results Tanshinones decreased ALT and MDA levels, and increased ORAC, vitamin C and GSH levels in liver tissues as compared with restraint stress control. Tanshinones also significantly inhibited oxidation in vitro. Among four tanshinones, dihydrotanshinone was more effective than others both in vivo and in vitro test. Conclusion Tanshinones possesses potent antioxidant activity in vitro and in vivo, and protected against liver injury induced by restraint stress. The active mechanisms may be related to their antioxidant capability.
出处
《药学学报》
CAS
CSCD
北大核心
2006年第7期631-635,共5页
Acta Pharmaceutica Sinica
关键词
丹参酮
拘束应激
氧自由基
肝损伤
tanshinone
restraint stress
oxygen radical
liver injury
