摘要
目的:探讨聪耳通窍汤加针刺治疗神经性耳鸣的临床疗效及作用机制。方法:选择我院耳鼻喉科、五官科神经性耳鸣患者93例,按随机数字表法分为针刺组(31例)、聪耳通窍汤组(31例)及针药合用组(31例),3组均治疗3个疗程,每个疗程为10 d,观察治疗前后患者血液中5-HT及Rho/ROCK信号通路的变化。结果:3个疗程后对患者治疗前后进行比较,针刺组有效率71%,中药复方组55%,针药合用组87.1%,其中针药合用组相对于其他2组差异有统计学意义;通过HPLC检测患者血液中5-HT发现,治疗后相对于治疗前差异有统计学意义,western blot检测Rho/ROCK信号通路发现,治疗后患者ROCK蛋白明显下降。结论:针药合用治疗神经性耳鸣差异有统计学意义,其治疗效果可能与抑制5-HT/Rho/ROCK信号通路有关。
Objective： To investigate the clinical efficacy and mechanism of Conger Tongqiao decoction added acupuncture in the treatment of neurological tinnitus. Methods： To choose ENT, ENT tinnitus patients, 93 patients were randomly divided into the acupuncture group （31 cases）, Conger Tongqiao decoction group （31 cases） and needle-drug combination group （31 cases）, the three groups were treated for three courses, each course is ten days, and to observe the patient＇s blood before and after treatment in serotonin （5-HT） and changes of Rho / ROCK signaling pathway. Results： After three courses of patients before and after treatment were compared, the effective rate of acupuncture was 71% , traditional Chinese medicine group was 55%, needle-drug combination group was 87. 1%, Where the needle drug combination group relative to the other two groups had significant difference; Patients＇ blood by HPLC detection of 5-HT found that after treatment with a very significant difference relative to pre-treatment, western blot detection of Rho/ROCK signaling pathway. It was found that after treatment the patient ROCK protein was significantly decreased by the detection of Rho/ROCK. Conclusion： The treatment on Acupuncture combined Conger Tongqiao Decoction of tinnitus had a very significant difference, its therapeutic effect is related to the inhibition of 5-HT/Rho/ROCK signaling pathway.
出处
《中国中医基础医学杂志》
CAS
CSCD
北大核心
2015年第5期581-583,587,共4页
JOURNAL OF BASIC CHINESE MEDICINE
