摘要
以引种栽培30～40年云杉树种为研究对象,通过对花粉进行生活力测定,从而分析并探讨引种云杉繁殖能力对环境的适应性特征,为今后开展杂交育种工作提供理论依据.文章采用4种花粉活性测定方法对6种云杉花粉进行活性测定试验,得出以下结果：4种方法中,I2-KI染色法和醋酸洋红染色法染色效果不佳,TTC染色法和离体培养法试验效果较好,用TT℃染色法和离体培养法对花粉进行活性测定,花粉活性均表现为白扦活性最高,粗枝云杉活性最低,且不同云杉花粉最适培养基中蔗糖浓度不同,多数以15 g/ml的蔗糖浓度萌发效果最好,而青海云杉以10 g/ml的蔗糖浓度萌发效果最佳.6种云杉花粉最适培养基中硼酸浓度均为0.05 g/ml.
With introduced 30 - 40 years Picea as research object, through determination of pollen activity, the adaptability of introduced Pi- cea to environment was analyzed and discussed, which will provide theoretical basis for carrying out hybrid breeding work. Four pollen activity determination methods were adopted to conduct experiments on six kinds of Picea pollen. The results showed tha among four kinds of methods, 12-KI staining and acetocarmine staining are poor test results, TIC staining and in vitro culture test are better. By TIC staining and in vitro culture method for measuring the activity of pollen, pollen activity showed P. meyeri is the highest, the lowest activity is P. asperata. And dif- ferent P/cea pollen optimum concentration of sucrose are different, most are 15 g/ml of the sucrose concentration test the best, however, P. crassifolia is 10 g/ml sucrose concentration test the best results. Six kinds of Picea pollen optimum concentration of boric acid in the culture medium was 0.05 g/ml.
出处
《安徽农业科学》
CAS
2015年第17期241-245,共5页
Journal of Anhui Agricultural Sciences
基金
内蒙古林业厅科技支撑项目(内林科研[2013]2号)
关键词
云杉花粉
活性
测定方法
Picea pollen
Activity
Assay method
