摘要
[目的]探索出一套较好的大麦总RNA提取方法.[方法]以大麦的叶片、茎秆和子粒为试验材料,探索各器官总RNA提取方法.[结果]所得产物的检验结果表明,总RNA具有整齐且清晰的28S rRNA、18S rRNA条带,A260/A280均介于1.8 ～2.0.将提取的叶片总RNA反转录成cDNA,RT-PCR检测PCR产物在1 500bp处.[结论]用此方法提取的大麦各器官总RNA质量较好,纯度较高,完整性较好,可用于进一步的分子生物学研究.
[ Objective ] The aim was to explore total RNA extraction methods for different organs of barley. [ Method ] Using barley leaves, stems and grains as the experimental materials , the total RNA extraction method of these organs were explored. [ Result] The detected results of these products showed that the total RNA had clear and neat stripes of 28S rRNA and 18S rRNA in the RNA electrephoresis. The ratios of A~/A2so were between 1.8 to 2.0. Leaves total RNA isolated by these methods were reverse transcribed and high quality cDNA were ob- tained. RT-PCR detected the product of PCR was in the 1 500 bp. [ Conclusion] These results showed that the quality of the extracted barley total RNA were good, high purity, good integrity, and were suitable for further research in molecular biology.
出处
《安徽农业科学》
CAS
2015年第17期33-34,37,共3页
Journal of Anhui Agricultural Sciences
基金
国家自然科学基金项目(30360307)
关键词
大麦
总RNA
提取
Barley
Total RNA
Extraction
