摘要
目的观察通心络胶囊对糖尿病周围神经病变小鼠的作用。方法 KK/Upj-Ay小鼠随机分为4组,模型组与高、中、低3个剂量通心络组(0.8,0.4,0.2 g·kg-1),另设C57BL/6小鼠为对照组。灌胃给药12周,测定运动神经传导速度(MNCV)和感觉神经传导速度(SNCV);实时定量荧光聚合酶链反应和蛋白质印迹法测定坐骨神经肿瘤坏死因子-α(TNF-α)、白介素-6(IL-6)、白介素-1β(IL-1β)和核因子-κB(NF-κB)表达。结果与模型组比较,通心络组的小鼠MNCV、SNCV明显增快(P<0.05,P<0.01),TNF-α、IL-6、IL-1β和NF-κB的表达显著下降(P<0.05,P<0.01)。结论通心络胶囊可抑制NF-κB通路,抑制糖尿病周围神经病变小鼠炎症因子表达,从而改善糖尿病周围神经病变。
Objective To investigate the effect of Tongxinluo capsule on diabetic peripheral neuropathy in mice .Methods KK/Upj -Ay mice were randomly divided into 4 groups,model, Tongxinluo low, middle, high doses groups （0.8, 0.4, 0.2 g· kg -1 ）.C57BL/6 mice were se-lected as control group.Mice were given intragastrically for 12 weeks. Motor nerve conduction velocity （ MNCV ） and sensory nerve conduction velocity （ SNCV） were detected.The expression of tumor necrosis factor alpha （ TNF -α） , interleukin 6 （ IL -6 ） and interleukin 1 beta （IL-1β） and nuclear factor kappa B （NF-κB） in sciatic nerve were examined by real time PCR and Western blot .Results Compared with model group , MNCV and SNCV were faster （ P〈0.05 , P〈0.01 ） .The TNF-α, IL-6 , IL-1βand NF-κB expression significantly were de-creased in Tongxinluo group （ P 〈 0.05 , P 〈 0.01 ） . Conclusion Tongxinluo capsule inhibits inflammatory cytokines expres-sion in diabetic peripheral neuropathy mice by inhibiting NF -κB pathway , to ameliorates diabetic peripheral neuropathy .
出处
《中国临床药理学杂志》
CAS
CSCD
北大核心
2015年第10期874-877,共4页
The Chinese Journal of Clinical Pharmacology
基金
"十二五"重大新药创制科技重大专项基金资助项目(2011ZX09101-004-02)
河北省中医药管理局科研计划基金资助项目(2014155)
关键词
通心络胶囊
糖尿病周围神经病变
炎症因子
NF-ΚB通路
Tongxinluo capsule
diabetic peripheral neuropathy
inflammatory cytokines
nuclear factor kappa B pathway
