摘要
为了研究黄粉虫纤溶活性蛋白(Tenebrio Fibrinolytic Proteins,TFP)的免疫原性及其抗血栓作用,本研究将纯化的TFP经皮下注射免疫小鼠,间接ELISA法检测小鼠血清中的抗体含量及抗体效价;小鼠灌胃TFP后,取血浆检测凝血酶时间(thrombin time,TT)、凝血酶原时间(prothrombin time,PT)、活化部分凝血活酶时间(activated partial thromboplastin time,APTT)、组织型纤溶酶原激活剂(tissue-type plasminogen activator,t-PA)和其抑制物(plasminogen activator inhibitor,PAI),角叉菜胶使小鼠尾部形成血栓,测量血栓形成长度.结果显示,TFP免疫小鼠后产生特异性抗体且随着免疫次数的增加,抗体含量逐渐升高,末次免疫后产生的抗体效价为1∶640;TFP可以显著延长TT,增强t-PA的活性,抑制PAI的活性,同时显著抑制由角叉菜胶引起的小鼠尾部血栓的形成.这说明TFP具有良好的免疫原性且具有一定的抗血栓作用.
To study the immuongenicity and anti-thrombotic effect of Tenebrio Fibrinolyric Proteins （ TFP） , mice were immunized with purified TFP by subcutaneous injection , then the antibody content and antibody titer were detected by the optimal ELISA method .Mice were gavaged with purified TFP , then thrombin time （ TT） , prothrombin time （ PT） , activated partial thromboplastin time （ APTT） , tissue-type plasminogen activator （ t-PA） and plasminogen activator inhibitor （ PAI） activity were detected .The results show that the specific antibody is found after TFP immunized mice and the antibody content in -creases when the number of immunization rises .The specific antibody titer is 1∶640 after the last immuni-zation .The TT of mice is remarkably prolonged after using TFP .The t-PA activity increases while the PAI activity decreases .Meanwhile the average thrombosis length of tail is significantly reduced .This shows that the TFP has good immunogenicity and anti-thrombotic effect .
出处
《广东工业大学学报》
CAS
2015年第2期144-148,共5页
Journal of Guangdong University of Technology
基金
国家自然科学基金资助项目(30772739)
2011年海口市重点科技计划项目(2011-0044)
关键词
黄粉虫
纤溶活性蛋白
免疫原性
抗血栓
tenebrio molitor
fibrinolytic proteins
immunogenicity
anti-thrombotic
