摘要
目的：建立高效液相色谱法测定五甘桑冲剂中五味子甲素含量的方法。方法应用甲醇超声提取五甘桑冲剂样品10min，提取液经浓缩后甲醇溶解；高效液相色谱法测定，色谱柱：Diamonsil－C18柱（250mm×4．6mm，5μm）；流动相：甲醇－水（65∶35）；柱温25℃，流速0．8mL？min －1，进样体积10μL。结果五味子甲素在0．06～0．42mg？ mL －1范围内线性关系良好，Y＝0．0414X ＋0．028，r＝0．9992，回收率为98．3％， RSD＝1．4％（n＝9）。结论所建方法便捷、专属性强，适用五甘桑冲剂中五味子甲素的含量测定。
OBJECTIVE To establish the quantitative determination method of the deoxyschizandrin contents of WuGanSang Granules.METHODS Sample of WuGanSang Granules was extracted for 10min with methanol,the concentrated extract was dissolved;the deoxyschizandrin contents of WuGanSang granules was determinated by HPLC.The column：Diamonsil-C18（250mm ×4.6mm,5μm）,mobile phase：methanol-water（65∶35）,column temper-ature：25℃,flow rate：0.8mL· min -1 ,Volume：10μL.ResuLts：The deoxyschizandrin curve was linear within a range of 0.06～0.42mg· mL-1,linear equation was Y=0.0414X＋0.028（r=0.9992）,the average recovery was 98.3%with RSD of 1.4%（n=9）.CONCLUSION This method is convenient,stable and could be used to assay the de-oxyschizandrin contents of WuGanSang Granules.
出处
《海峡药学》
2015年第4期61-63,共3页
Strait Pharmaceutical Journal
