摘要
催化动力学分光光度法测定饲料中碘具有分析成本低、灵敏度高等优点,但该方法的测量误差较大,测量结果不易重现等缺陷影响了它在生产中的推广应用。为快速准确地测定饲料中的碘含量,有必要对整个实验过程中主要不确定度来源进行分析,明确关键控制环节,有效减少误差,为评价试验中测量结果的真确性提供科学依据。建立测量过程中主要分量的定量数学模型,通过最小二乘法线性拟合曲线,对数学模型中的定容体积、试样称量、重复测定等分量进行不确定度量化,合成得到了测定结果的相对不确定度及扩展不确定度。在95%的置信概率下,碘含量的测量结果报告为X=(2.48±0.29)mg/kg,k=2。影响催化动力学分光光度法测定饲料中碘含量的不确定度主要来源是由标准系列配制和消化液定容体积所产生的不确定度。对这两个环节加强质量控制,即可减少误差,提高测定结果的准确度。
Determination of iodine in feeds by catalytic kinetic spectrometry has the advantages of low cost,and high sensitivity,but the measurement error of this method is large,the measurement results are not easy to reproduce,which affects its application in production.In order to determine iodine content in feed quickly and accurately,it is necessary to analyze the main sources of uncertainty in the whole process of the experiment,clarify the key control links,effectively reduce the error,and provide scientific basis for the authenticity of the measurement results of the evaluation experiment.The quantitative mathematical mode of respective components in experiment was established,fitting curve by the least squares method,the relative and expanded uncertainty of volume size,sample weighing and repeated measurements were analyzed and calculated by the model.At the 95% confidence probability,the measurement results for iodine content reported as X=（2.48±0.29）mg/kg,k=2.Standard series preparing and volume of digestive fluid were the main sources of uncertainty.Strengthening the quality control of the two links could reduce the error,and improve the measurement accuracy.
出处
《粮食与饲料工业》
CAS
2015年第5期65-69,共5页
Cereal & Feed Industry
基金
新疆农科院农产品质量安全重点实验室建设项目(xjnkkl-2013-003)
2014年国家特色农产品质量安全风险评估项目(GJFP2014008)
关键词
催化动力学分光光度法
饲料
碘含量测定
不确定度评定
catalytic kinetic spectrophotometric
feed
determination of iodine content
uncertainty evaluation
