摘要
为了研究五指山猪遗传多样性和系统地位,本研究采用PCR产物直接测序法得到55条五指山猪线粒体控制区(mtDNA D-Loop)全序列,并结合GenBank中发表的1条五指山猪mtDNA D-Loop全序列进行结构分析、遗传多样性研究及系统发育分析。结果发现,56条序列中出现21次保守区变异,即"TTATAAAACAC"序列重复,共定义13个单倍型,发现14个变异位点,单倍型多样度(Hd)和核苷酸多样度(Pi)分别为0.838和0.00334,表明五指山猪遗传多样性较丰富。五指山猪线粒体控制区序列构建的系统发育树和Network网络分析呈现两大分支,推测有两个母系起源,且与GenBank中其他23个猪种的聚类结果表明,五指山猪与长江流域以南地区的猪种有着较近的亲缘关系。
In order to research the genetic diversity and phylogenies of Wuzhishan pig,56 complete mtDNA D-Loop were analyzed. 55 sequences were amplified by polymerase chain reaction （PCR） and 1 sequence was downloaded from GenBank. The results showed that repetitive sequence ＂TTATAAAACAC＂ appeared in the conservative regions of 21 mtDNA D-Loop sequences. 13 haplotypes and 14 variable sites were observed in 56 sequences. Haplotype diversity and nucleo- tide diversity （Hd =0. 838 and Pi=O. 00334） indicated that Wuzhishan pig had high genetic di- versity. Two branches in the phylogenetic tree and Network analysis diagram indicated that there were two maternal origins in Wuzhishan pig. The phylogenetic tree of Wuzhishan pig and other 23 species showed that Wuzhishan pig had close genetic relationship with the pig breeds in the south region of the Yangtze river.
出处
《中国畜牧兽医》
CAS
北大核心
2015年第5期1222-1231,共10页
China Animal Husbandry & Veterinary Medicine
基金
家养动物种质资源平台运行服务
中央级公益性科研院所基本业务费(1630032013020)
关键词
五指山猪
线粒体控制区
遗传多样性
Wuzhishan pig
mitochondria
DNA D-Loop
genetic diversity
