摘要
【目的】利用mtDNA CytB基因为标记,从分子水平探讨12个猪种166个样本(8个山东省猪种,3个引进猪种和梅山猪种)的遗传多样性和各猪种间的亲缘关系,为山东省猪种合理利用以及生物多样性保护提供基础资料。【方法】对各猪种样本mtDNA CytB基因进行PCR扩增和测序,采用现代生物信息学方法进行数据处理分析。【结果】山东8个猪种种内遗传变异和种间遗传距离均较小,有共享单倍型。山东各猪种和梅山猪与大约克的遗传距离也较小,但与引进猪种杜洛克和长白的遗传距离较大。利用分子系统进化树和CytB基因4个SNP位点的分类方法将上述样本分为2个独立的支系,第一支以中国猪种为主,将除鲁烟白猪的一种单倍型在内的所有山东猪种、梅山猪和大约克的两种单倍型聚为一类,第二支以引进猪种为主,将长白、杜洛克、大约克的一种单倍型和鲁烟白猪的一种单倍型聚为一类。【结论】山东猪种有共同的母系祖先,品种内母系遗传多样性较贫乏;外来猪种引入山东后主要是用作父本以提高生产速度和瘦肉率等,对本地猪种未有母系贡献;利用CytB基因4个SNP位点对欧洲单倍型和亚洲单倍型的分类方法,可以方便地估测利用中外猪种结合培育的猪新品种(配套系)的母系血统情况。
[Objective] In order to provide data and scientific basis for reasonable utilization and protection ofbiodiversity, the genetic diversity and phylogenetic relationships of 12 pig breeds （8 pig breeds from Shandong Province, 3 introduced pig breeds and Meishan pigs） were studied. [Method] In the study, the PCR amplification, sequencing and data analyzing ways of biologic informatics were used. [Result] All the 8 pig breeds from Shandong Province shared common haplotype, had rather small genetic diversity within breeds and small mean genetic distances between breed too. The pig breeds from Shandong Province had rather small mean genetic distances with Meishan and Yorkshire too, but had big distances with Duroc and Landrance. Both the NJ phylogenetic tree and the classified measure based on four SNPs of CytB, which classify the pigs into European and Asian haplotypes, classified the above samples into 2 clades. One included all sample of pig breeds from Shandong Province excepted one haplotype of Luyan White pigs, Meishan pigs and two haplotypes of Yorkshire, and the other included Duroc, Landrace, part haplotype of Yokshire and one haplotype of Luyan White pigs. [Conclusion] Firstly, the pig breeds from Shanaong Province had the common maternal ancestor, the genetic diversity were rather small within every breeds. Secondly, the introduced pigs were used as sire, not dam, to improve the growth performance and lean meat percentage of the pig breeds from Shandong Province. Thirdly, it was a convenient measure to use the 4 SNPs of CytB to detect the maternal blood of the new pig breeds （or synthetic lines） bred by Chinese and European pig breeds.
出处
《中国农业科学》
CAS
CSCD
北大核心
2009年第5期1761-1767,共7页
Scientia Agricultura Sinica
基金
山东省农业科学院创新基金(2007YCX016)
山东省农业良种工程-优质肉猪配套系选育(2006LZ08)
畜禽种质资源描述标准与共享平台建设(2005DKA21101)
关键词
山东省猪种
MTDNA
CYTB
遗传多样性
系统进化
pig breeds from Shandong Province
mtDNA
CytB
genetic diversity
phylogenetic relationship
