摘要
对紫蛇尾Ophiopholis mirabilis皂苷粗提物进行初步纯化,并对所得产物的抑菌活性、抑制α-葡萄糖苷酶活性和细胞毒活性进行了研究。采用AB-8大孔吸附树脂初步纯化紫蛇尾皂苷粗提物,采用香草醛—高氯酸法测定总皂苷含量,采用滤纸片法测定抑菌活性,采用比色法和MTT法分别测定α-葡萄糖苷酶和MCF-7人乳腺癌细胞的抑制率。结果表明:纯化后的紫蛇尾皂苷纯度增加至48.57%;皂苷粗提物及纯化物的抑菌活性表现一致,均对枯草芽孢杆菌Bacillus subtilis具有明显的抑制作用,且纯化物的抑菌效果略强;皂苷粗提物对α-葡萄糖苷酶的抑制效果明显,大孔树脂95%乙醇洗脱所得纯化物的抑制率达到94.62%;皂苷粗提物及纯化物对MCF-7人乳腺癌细胞均表现出了很强的细胞毒活性,其中大孔树脂60%、75%、95%乙醇洗脱所得纯化物的浓度为300μg/m L时,细胞抑制率均高达97%以上。研究表明,紫蛇尾皂苷粗提物及纯化物均表现出了很强的选择性抑菌活性、α-葡萄糖苷酶抑制活性和对MCF-7人乳腺癌细胞的细胞毒活性,且纯化物的活性增强。
Saponins in brittle star Ophiopholis mirabilts were prenmmamy purlneu tJy ~D--O mu^lup total content of saponins were determined by vanillin-perchloric acid method. Antibacterial activity and inhibitory effect on alpha glucosidase activity, and cytotoxic activity were tested by filter paper method, colorimetric method and MTT method. The results showed that the purity of the saponins was increased to 48.57% after purification by AB-8 macro porous resin. The antibacterial manifestations of crude extracts and purified saponins showed the same antibacterial activity, especially strong inhibition on Bacillus subtilis. However, the purified saponins had higher antibacterial effects, significant inhibitory effect on alpha glucosidase activity, with inhibition rate of 94.62% in 95% ethanol elution fractions. Moreover, both crude extracts and purified saponins showed significant cytotoxic ac- tivity of MCF-7 human breast cancer ceils. The inhibition rate of over 97% were observed in 60%, 75%, and 95% ethanol elution fractions at 300 μg/mL. Both of crude extracts and purified saponins showed strong alternative inhibition on Bacillus subtilis, strong inhibitory effect on alpha glucosidase activity and cytotoxic activity of MCF-7 human breast cancer cells.
出处
《大连海洋大学学报》
CAS
CSCD
北大核心
2015年第2期221-225,共5页
Journal of Dalian Ocean University
基金
国家海洋公益性行业科研专项(201205022-7)
关键词
紫蛇尾皂苷
抑菌
抑制α-葡萄糖苷酶
细胞毒活性
Ophiopholis mirabilis saponin
antibacterial activity
inhibition of alpha glucosidase
cytotoxic activity
