摘要
目的探讨长春西汀对小鼠肾缺血再灌注(IR)损伤的保护作用及其作用机制。方法将48只健康小鼠分成假手术组、IR组、长春西汀低剂量干预组和长春西汀高剂量干预组,采用双侧肾蒂夹闭45min的方法建立小鼠肾IR损伤模型,HE染色后观察肾脏组织形态学变化,测定再灌注24h后各组血肌酐(SCr)、尿素氮(BUN)、肿瘤坏死因子(TNF-α)和白细胞介素-1β(IL-1β)和IL-6水平,观察肾脏组织中超氧化物歧化酶(SOD)活性和丙二醛(MDA)水平。结果与假手术组相比,IR组出现肾小管管腔扩张,刷状缘消失,管腔内有管型等病理改变,血清中SCr、BUN、TNF-α、IL-1β和IL-6水平明显升高,差异均有统计学意义(P<0.05),肾组织SOD活性降低,差异有统计学意义(P<0.05),MDA水平升高,差异也有统计学意义(P<0.05);与IR组相比,长春西汀10mg/kg和20mg/kg干预可呈剂量依赖性改善肾组织病理损伤,SCr、BUN、TNF-α、IL-1β、IL-6和MDA水平减少,使肾组织SOD活性升高,差异有统计学意义(P<0.05)。结论长春西汀可通过抑制炎症和氧化应激来减轻肾IR损伤。
Objective To investigate the protective effect and mechanism of vinpocetine in renal ischemia‐reperfusion injury .Methods Forty‐eight healthy male mice were randomly divided into the sham operation group ,is‐chemia‐reperfusion （IR） group ,low dose and high dose（10 mg/kg and 20 mg/kg） vinpocetine pretreatment groups . The renal ischemia‐reperfusion injury models were established by clamping bilateral renal pedicle for 45 min .The re‐nal morphology changes were observed by the HE staining .The Scr ,BUN ,TNF‐α,IL‐1βand IL‐6 levels at 24 h after reperfusion were detected .The superoxide dismutase （SOD） activity and malondialdehyde （MDA） contentrations were determined .Results Compared with the sham operation group ,the pathological changes in the IR group were tubular dilatation ,loss of brush border and casts in lumen .The levels of Scr ,BUN ,TNF‐α,IL‐1βand IL‐6 in the IR group were significantly increased（P〈0 .05） .The SOD activity of kidney tissue was significantly decreased and the MDA contentration was increased ,the differences were statistical significant（P〈0 .05）;compared with the IR group , vinpocetine10 mg/kg and 20 mg/kg could improve the pathological injury of renal tissue in a dose‐dependent manner , the levels of Scr ,BUN ,TNF‐α,IL‐1β,IL‐6 and MDA were decreased and the SOD activity was increased ,the differ‐ences were statistically significant（P〈0 .05） .Conclusion Vinpocetine can ameliorate the renal ischemia‐reperfusion injury in mice by inhibiting inflammation and oxidative stress .
出处
《检验医学与临床》
CAS
2015年第9期1183-1184,1187,共3页
Laboratory Medicine and Clinic
基金
国家自然科学基金(31071262)
关键词
长春西汀
肾缺血再灌注损伤
炎症因子
氧化应激
vinpocetine
renal ischemia reperfusion
inflammatory factor
oxidative stress
