摘要
目的 研究益智防呆方缓解Aβ细胞毒性的作用与机制.方法 益智防呆方含药血清干预Aβ处理的人神经母细胞瘤细胞（SH-SY5Y细胞）,运用MTT法检测细胞存活率,免疫印迹法检测Hsp70、Grp78及Caspase-3蛋白表达.结果 益智防呆方含药血清可提高经Aβ处理后的细胞存活率,降低凋亡相关蛋白Caspase-3表达,促进Hsp70蛋白表达增加,激活内质网应激反应.结论 益智防呆方可有效缓解Aβ细胞毒性,可能与Hsp70的过表达和激活内质网应激有关.
Objective To investigate the protective effects and mechanisms of YiZhiFangDai formula in Aβ 1-42-induced neurotoxicity in SH-SYSY cells. Methods Aβ1-42-treated-SH-SYSY cells were pretreated with serum that treated with YiZhiFangDai formula. Cell viability was detected by using MTT. The expression of Hsp70, Grp78 and Caspase-3 were measured by using western blotting. Results In contrast to Aβ1-42-treated group, the pretreatment of YizhiFangDai formula significantly decrease the neurotoxicity of Aβ1-42, increase the expression Hsp70 and Grp78, and decreased Caspase-3 ex- pression in a dose-dependent manner. Conclusion YizhiFangDai formula may attenuate Aβ1-42 induced neurotoxicity by affecting the expression of Hsp70 and endoplasmic reticulum stress.
出处
《老年医学与保健》
CAS
2015年第2期87-89,共3页
Geriatrics & Health Care
基金
国家自然科学基金(81473739)
上海市科委中药现代化专项基金项目(12401904500)
