摘要
通过形态学和分子生物学分析,实验菌株为药用拟层孔菌。采用不同有机溶剂提取药用拟层孔菌子实体,分别获得石油醚、氯仿、乙酸乙酯和甲醇提取物。采用GC-MS方法,分析药用拟层孔菌石油醚提取物中的化学成分,同时对不同有机溶剂提取物的生物活性进行检测。结果表明:药用拟层孔菌石油醚提取物中共鉴定出46个化合物。体外抑制肿瘤细胞活性实验表明,在100μg/m L时,不同有机溶剂提取物都表现出抑制肿瘤细胞活性。其中,石油醚提取物在50μg/m L时,其对NCI-H460和SGC-7901肿瘤细胞的抑制率分别为99.03%和82.57%。甲醇提取物在8mg/m L时,DPPH自由基清除率达93.35%。
The fruiting bodies of Fomitopsis officinalis were extracted, using different polarity solvents. Petroleum ether extract,chloroform extract, ethyl acetate extract and methanol extract were obtained. The constituents of petroleum ether extracts were investigated by GC-MS, and the biological activities of extracts in different phase were measured. Forty-six kinds of consti- tuents were identified from the petroleum ether extract. The extracts obtained by using the four solvents showed anti-tumor activity at 100μg/mL. The petroleum ether extract components at 50μg/mL displayed strong growth-inhibitory effects on NCI-H460 and SGC-7901 tumor cells with 99.03% and 82.57% of inhibition rate, respectively. The free radical scavenging rate ofmethanol extracts at 8mg/mL was 93.35%.
出处
《菌物学报》
CAS
CSCD
北大核心
2015年第3期482-489,共8页
Mycosystema
关键词
药用拟层孔菌
抗肿瘤
抗氧化
Fomitopsis officinelis, anti-tumor, antioxidant
