摘要
目的:探讨布地奈德对哮喘大鼠模型气道重塑及平滑肌中Toll样受体4表达的影响。方法:将30只SPF级SD大鼠随机分为支气管哮喘组(A组)、健康对照组(B组)、布地奈德治疗组(C组)3组,每组10只。制备大鼠肺组织病理切片,HE染色后观察各组气道结构改变情况,并测定支气管壁厚度(WAt/Pi)、支气管壁平滑肌厚度(WAm/Pi)及支气管平滑肌细胞核数(N/Pi),利用Western-Blot方法检测气道平滑肌中TLR4的蛋白表达。结果:光镜下可见A组支气管上皮细胞脱落、管壁炎症细胞浸润、杯状细胞增生、基底膜和平滑肌明显增厚,而C组上述改变较A组明显减轻。与B组比较,A组支气管管壁厚度、平滑肌厚度及平滑肌细胞核数显著升高(P<0.01),与A组比较,C组支气管管壁厚度、平滑肌厚度及支气管平滑肌细胞核数显著降低(P<0.01)。A组的TLR4的蛋白水平明显高于B组及C组(P<0.01)。结论:吸入布地奈德可以防治气道重塑,可能通过抑制TLR4的表达实现。
Objective ：To explore the influence of budesonide on the airway remodeling and TLR4 of airway smooth muscle cell in Rat models of asthma .Methods ：Thirty SD rats were randomly divided into 3 groups ：model group（group A） ,normal control group（group B） ,budesonide treatment group（group C） ,with 10 rats in each group . The airway inflammation and the alteration of airway structure were detected by HE staining ,The airway wall thick‐ness（WAt/ Pi） ,the bronchial smooth muscle thickness（WAm/Pi）and numbers of smooth muscle cell （N /Pi ）were measured by using image analysis system .the expression of TLR4 protein in cells were detected by Western blot .Results ：Bronchial epithelial desquamation ,inflammatory cell infiltration ,hyperplasia of goblet cells and smooth muscle cells were seen in group A under optical microscope ,however compared with group A ,the manifestations were signif‐icantly decreased in group C .Compared with group B ,WAt/ Pi ,WAm/Pi and N/Pi were expressed at higher levels in lung tissues of group A（P 〈 0 .01） .Compared with group A ,WAt/ Pi ,WAm /Pi and N /Pi were expressed at lower levels in lung tissues of group C（P〈 0 .01） .Compared with group B and C ,the expression of TLR4 at protein levels in group A was significantly highter .Conclusion ：The airmay remodeling can be inhibited by budesonide proba‐bly through reducing the expression of TLR4 .
出处
《陕西医学杂志》
CAS
2015年第5期524-526,共3页
Shaanxi Medical Journal
