摘要
为探讨高糖和脂多糖(LPS)刺激下人脐静脉内皮细胞(HUVEC)TLR4的表达及高糖促进动脉粥样硬化与TLR4的关系,我们用RT-PCR和流式细胞术分别测定了高糖和LPS刺激下HUVECTLR4 mRNA和蛋白的表达;用ELISA测定高糖和LPS刺激下HUVECMCP-1的产生。研究结果表明,高糖上调HUVECTLR4 mRNA及其蛋白的表达,促进HU-VEC分泌MCP-1;高糖协同LPS上调HUVEC细胞TLR4蛋白的表达,使HUVEC分泌MCP-1进一步增加。研究发现,高糖促进动脉粥样硬化与TLR4有关;高糖可能协同感染因素促进动脉粥样硬化的发生。
To explore the effect of high level of glucose and lipopolysaccharide（LPS） on the expression of Toll-like receptor 4（TLR4） in human umbillical venous endothelial cells（HUVEC）,the HUVEC were stimulated with high level of glucose and LPS,and then RT-PCR analysis and flow cytometry assay were used to measure the expression of TLR4 mRNA and protein on these cells.The concentration of MCP-1 was measured with ELISA. The results showed that high level of glucose up-regulated the gene and protein expression of TLR4 in HUVEC and promote the secretion of MCP-1 protein from these cells. High level of glucose co-effecting with high level of LPS.Also up-regulate the expression of TLR4 protein on these cells to induce the further secretion of MCP-1 protein. The results of our study suggest that the mechanism that high level of glucose accelerates the course of atherosclerosis may be related with the expression of TLR4,and possibly high level of glucose may co-effect with the infectious factors to induce the development of atherosclerosis.
出处
《现代免疫学》
CAS
CSCD
北大核心
2010年第4期324-327,共4页
Current Immunology
关键词
高糖
TOLL样受体4
动脉粥样硬化
脂多糖
high glucose
Toll-like receptor 4
atherosclerosis
lipopolysaccharide
