摘要
本研究以优良品种绿宝石梨的嫩茎稍为试材,对影响组织培养的主要因素,包括培养基种类、生长调节剂浓度配比进行了研究,建立了绿宝石梨的组织快繁技术。试验结果表明,适宜绿宝石梨组织培养的启动培养基由MS、1.0mg/L 6-BA、0.1mg/L IBA组成;增殖培养基是由1/2MS、1.0mg/L 6-BA、0.1mg/L IBA组成。
In this paper, tender stems of Lvbaoshi pear were used as test material. Effects of media types, 6-BA and IBA with different concentrations on Lvbaoshi pear issue culture were studied, which established the fast-breeding technique of Lvbaoshi pear. The results showed that the suitable culture medium for different stages were initial medium and multiplication medium. Initial medium included MS, 1.0mg/L 6-BA, and 0.1mg/L IBA. Multiplication medium included 1/2MS, 1.0mg/L 6-BA, and IBA 0.1mg/L.
出处
《中国果菜》
2015年第4期49-51,共3页
China Fruit & Vegetable
基金
河南省教育厅自然科学研究计划项目(2010C210001)
河南省高等学校青年骨干教师资助计划项目(2013GGJS-252)
关键词
绿宝石梨
嫩茎
组织培养
Lvbaoshi pear
tender stems
issue culture
