摘要
目的:检测去势大鼠阴茎海绵体中血红素加氧酶2(HO-2)/CO的表达,初步探讨勃起功能障碍(ED)的发病机制。方法:雄性SD大鼠72只均分为对照组、假手术组、去势组和去势锌原卟啉(Zn PP,HO阻断剂)组,检测基础条件和阿扑吗啡(APO)刺激后的海绵体内压(ICP)和勃起率;激光共聚焦显微镜检测大鼠勃起不同时期阴茎海绵体组织中HO-2蛋白的表达,分光光度计测定CO在大鼠勃起不同时期的含量。结果:基础条件和APO刺激后ICP和勃起率,去势组[(11.68±0.69)mm Hg,(54.81±3.86)mm Hg,33.3%]和去势Zn PP组[(11.20±0.71)mm Hg,(41.17±5.41)mm Hg,22.2%],与对照组[(22.83±2.66)mm Hg,(66.92±7.77)mm Hg,100%]和假手术组[(23.35±2.22)mm Hg,(70.43±7.22)mm Hg,100%]比较显著下降(P均<0.01),且APO刺激后去势Zn PP组ICP较去势组明显降低(P<0.01)。APO刺激后,勃起前和勃起中阴茎海绵体HO-2蛋白表达,去势组(445.4±23.7,847.4±35.0)和去势Zn PP组(390.1±29.7,526.0±52.5)较对照组(512.7±57.4,1 145.2±89.8)和假手术组(583.7±8.0,1 016.3±79.8)显著下降(P<0.05或P<0.01),且勃起中去势Zn PP组HO-2蛋白表达较去势组明显降低(P<0.01);勃起后各组之间的HO-2蛋白表达变化差异无显著性。勃起前、中和后阴茎海绵体CO含量,去势组[(20.59±1.01)×10-7nmol/L,(32.53±1.26)×10-7nmol/L,(18.71±1.22)×10-7nmol/L]和去势Zn PP组[(12.52±1.05)×10-7nmol/L,(21.90±1.02)×10-7nmol/L,(16.56±0.55)×10-7nmol/L]较对照组[(26.76±1.41)×10-7nmol/L,(48.25±1.01)×10-7nmol/L,(27.10±1.58)×10-7nmol/L]和假手术组[(25.41±2.09)×10-7nmol/L,(47.90±1.22)×10-7nmol/L,(25.67±1.20)×10-7nmol/L]显著下降(P<0.05或P<0.01),且勃起中去势Zn PP组CO含量较去势组明显下降(P<0.01)。结论:HO-2和CO表达下降与去势大鼠ED的发生有关。
Objective：To explore the expressions of HO-2 and CO in the corpus cavernosum of castrated rats in order to further study the pathogenesis of erectile dysfunction（ ED）.Methods：We randomly divided 72 male SD rats into four groups：normal control,sham operation,castration,and castration ＋ Zn PP.We detected intracavernous pressure（ ICP） and penile erection in the basic condition and after apomorphine（ APO） induction,determined the expression of the HO-2 protein in the corpus cavernosum by laser scanning confocal microscopy,and measured the level of CO by spectrophotometry during different periods of penile erection.Results：The ICP in the basic condition and that after APO induction and the rate of penile erection were decreased significantly in the castration group（ [11.68 ± 0.69] mm Hg,[54.81 ± 3.86] mm Hg,and 33.3%） and the castration ＋ Zn PP group（[11.20 ± 0.71]mm Hg,[41.17 ± 5.41] mm Hg,and 22.2%） as compared with the normal control（[22.83 ± 2.66] mm Hg,[66.92 ± 7.77] mmHg,and 100%） and the sham operation group（[23.35 ± 2.22]mm Hg,[70.43 ± 7.22]mm Hg,and 100%）（ all P 〈0.01）.After APO induction,ICP in the castration ＋ Zn PP group was remarkably reduced in comparison with that in the castration group（ P 〈0.01）,and so was the expression of the HO-2 protein before and during penile erection in the castration（445.4 ± 23.7 and 847.4 ±35.0） and the castration ＋ Zn PP group（ 390.1 ± 29.7 and 526.0 ± 52.5） compared with the normal control（ 512.7 ± 57.4 and1145.2 ± 89.8） and the sham operation group（583.7 ± 8.0 and 1016.3 ± 79.8）,the expression of the HO-2 protein significantly decreased in the castration group（445.4 ± 23.7 and 847.4 ± 35.0）（ P 〈0.05 or 0.01）,markedly lower in the castration ＋ Zn PP than in the castration group during penile erection（ P 〈0.01） but with no significant differences among the four groups after it.Before,during and after penile erection,the levels of CO were remarkably decreased in the cas
出处
《中华男科学杂志》
CAS
CSCD
北大核心
2015年第5期396-401,共6页
National Journal of Andrology
基金
黑龙江省自然科学基金(D201118)~~
