摘要
目的:探讨基于肺虚络瘀病机理论中药复方颗粒剂对特发性肺纤维化(IPF)大鼠肺组织转化生长因子-β1(TGF-β1)蛋白及m RNA表达的影响。方法:120只Wistar大鼠按体质量数字表随机分为正常对照组、模型组、泼尼松组、参龙煎剂颗粒剂组,采用气管内注入博莱霉素建立IPF模型。造模后第2天起泼尼松组和参龙煎剂颗粒剂组按一定配比剂量灌胃,正常对照组和模型组每日予等容0.9%氯化钠溶液灌胃,1次/d。并分别于第7、14、28天取材,采用免疫组化、逆转录-聚合酶链式反应检测TGF-β1蛋白及m RNA的表达。结果:博来霉素造模后第7天,各组TGF-β1及TGF-β1 m RNA在大鼠肺组织呈广泛表达,为3个时间点表达最高,且随时间动态变化表达逐渐下降。同一时间点比较,模型组大鼠TGF-β1及TGF-β1 m RNA在肺组织表达始终高于泼尼松组和参龙煎剂颗粒剂组(P<0.01,P<0.05),表达最少的是正常对照组(P<0.01);用药干预治疗组比较,随着时间的推移,TGF-β1及TGF-β1 m RNA在大鼠肺组织呈逐渐递减趋势,28d时,参龙煎剂颗粒剂组与泼尼松组TGF-β1蛋白表达相比有统计学差异(P<0.05)。结论:参龙煎剂可能通过抑制TGF-β1蛋白和m RNA的高表达,改善肺组织损伤愈合与纤维化过程。
Objective： To investigate the influence of traditional Chinese medicine compound granula on protein and mRNA expression of TGF-β1 in lung tissue of rats with idiopathic pulmonary fibrosis （IPF） based on pathogenesis theory of Feixu Luoyu. Methods： One hundred and twenty Wistar rats were randomly divided into control group, model group, prednison group, Shenlong Jianji （SLJJ） group according to the random number table. Rat models with IPF were established by using intratracheal injection of bleomycin. Since the second day, control group and model group received gavage administration with normal saline, and the other two groups received gavage administration with equal quantity of corresponding drug. The rats were sacrificed on the 7th, 14th, and 28th day. Immunohistochemical staining and reverse transcription polymerase chain reaction were used to test the protein and mRNA expression of TGF- β1. Results： On the 7th day after rat model building, the protein and mRNA expression of TGF-β1 were widely expressed in rats lung tissues of each group, and the highest expression was at 3 time points,and the expression gradually declined with the dynamic change of time. At the same time point, the protein and mRNA expression of TGF- β1 of rats in model group was higher than that of predinison group and SLJJ group （P〈0.01, P〈0.05）, and protein and mRNA expression of TGF- β1 of rats in control group was the least （P〈0.01）. As time goes on, the protein and mRNA expression of TGF- β1 of rats in SLJJ group showed a gradually decreasing trend. On the 28th day, the difference in expression of TGF-β1 protein between SLJJ group and predinison group was significant （P〈0.05）. Conclusion： SLJJ could improve lung tissue damage and fibrosis through inhibiting the high expression of protein and mRNA expression of TGF-β1.
出处
《中华中医药杂志》
CAS
CSCD
北大核心
2015年第5期1560-1565,共6页
China Journal of Traditional Chinese Medicine and Pharmacy
基金
国家自然科学基金面上项目(No.81373579)
国家自然科学基金青年基金项目(No.81403290)
"十二五"国家中医药管理局中医络病重点学科建设项目
辽宁省科技厅计划项目(No.2012225018-202)
辽宁省中医临床重点学(专)科服务能力建设项目(No.2013-LNZYXZK-2)
辽宁省高等学校科学研究一般项目科学基础研究(No.L2014369)
沈阳市科技局计划项目(No.F14-231-1-13)~~
