摘要
目的:通过观察冠心康对骨髓内皮祖细胞(EPC)的增殖、迁移和黏附能力的影响,探知冠心康抗动脉粥样硬化(AS)的作用机制与SDF-1/CXCR4生物轴之间的关系。方法:36只8周龄雄性Apo E-/-小鼠分为模型对照组、AMD3100组和冠心康组;12只8周龄近交系C57BL/6/J小鼠作为正常对照组。各组于治疗12周时取材,采用HE染色检测各组小鼠主动脉横截面病理损伤程度;MTT比色法检测EPC增殖能力,Transwell迁移法检测迁移能力,EPC黏附实验检测EPC黏附能力;RT-PCR和Western blotting分别检测各组骨髓源EPC CXCR4 m RNA和蛋白表达。结果:冠心康可抑制骨髓源EPC的增殖、迁移、黏附,冠心康组小鼠骨髓源EPC的增殖、黏附和迁移能力明显低于模型对照组(P<0.05),冠心康组骨髓源EPC CXCR4 m RNA和蛋白表达水平明显低于模型对照组(P<0.01),冠心康组小鼠主动脉AS程度明显轻于模型对照组(P<0.05)。结论:中药复方冠心康可抑制骨髓源EPC的增殖、迁移、黏附,抑制骨髓源性EPC CXCR4基因和蛋白表达的作用,可能与冠心康调控SDF-1/CXCR4生物轴有关。
Objective： To investigate the effects of Guanxinkang on proliferation, migration and adhesion ability of bone marrow derived endothelial progenitor cells （EPC）, so as to reveal the correlation between SDF-1/CXCR4 biological axis and anti-atherosclerosis function of Guanxinkang. Methods： Thrity-six male ApoE-/- mice were randomly divided into model group, AMD3100 group and Guanxinkang group, and 12 inbred line C57BL/6 mice were set as control group. Mice were sacrificed and tissues were collected on the 12th week. HE staining method was used to observe the pathological damage degree of aortic cross section of mice in each group. MTT method was used to test the proliferation ability of endothelial progenitor cells. Transfer ability was determined by using Transwell method, and adhesive capacity of EPC was determined by using endothelial progenitor cell adhesion experiments. RT-PCR and Western blotting were used to test mRNA and protein expression of CXCR4 in EPC. Results： Guanxinkang could inhibit the proliferation, migration and adhesion abilities of EPC, and the proliferation, migration and adhesion abilities of EPC in Guanxinkang group were lower than that of model group （P〈0.05）. The mRNA and protein expression of CXCR4 in EPC of Guanxinkang group was lower than that of model group （P〈0.01）, and the degree of aortic atherosclerosis of mice in Guanxinkang group was lighter than that of model group （P〈0.05）. Conclusion： Guanxinkang could inhibit proliferation, migration, adhesion and the gene and protein expression of CXCR4 of EPC, which might be related with the regulation effect of Guanxinkang on SDF-1/CXCR4 biological axis.
出处
《中华中医药杂志》
CAS
CSCD
北大核心
2015年第5期1551-1555,共5页
China Journal of Traditional Chinese Medicine and Pharmacy
基金
2012年度上海市优秀学术带头人计划项目(No.12XD1404700)
教育部博士点基金资助项目(No.20133107110003)
上海高级中西医结合人才培养项目(No.ZYSNXD012-RC-ZXY008)~~
