摘要
目的:观察冠心康对小鼠主动脉及血管内皮细胞miRNA-126及其下游相关信号GRS16、CXCL12、CXCR4、VCAM-1表达的调控,探索中医药抗动脉粥样硬化的作用机制。方法:体外细胞实验中,将小鼠血管内皮细胞随机分为5组,即对照组、模型组、中药高剂量含药血清组、中药低剂量含药血清组。不同组别VEC分别经不同含药血清干预后,观察细胞增殖和凋亡情况,采用qRT-PCR和Western-blot检测VEC中pri-miR-126、RGS16、CXCL12、CXCR4、VCAM-1 mRNA和蛋白表达;体内实验采用HE染色检测各组小鼠主动脉横截面病理损伤程度,采用qRT-PCR和Western-blot检测主动脉pri-miR-126、RGS16、CXCL12、CXCR4、VCAM-1 mRNA和蛋白表达。结果:体外实验结果显示,与对照组相比,中药高低剂量组的VEC凋亡率和增殖率明显优于模型组(P均<0.05),中药高剂量组和中药低剂量组VEC增殖率比较差异不明显(P>0.05);与对照组相比,中药高低剂量组VEC中miR-126、RGS16、CXCL12、CXCR4和VCAM-1 mRNA表达明显优于模型组(P<0.05),中药高剂量组和中药低剂量组间比较差异不明显(P>0.05);与对照组相比,中药高低剂量组VEC中RGS16、CXCL12、CXCR4和VCAM-1蛋白表达明显优于模型组(P<0.05),中药高剂量组和中药低剂量组间比较差异不明显(P>0.05)。体内实验结果显示,小鼠主动脉HE染色结果,对照组小鼠主动脉血管管径厚薄均匀,内膜光滑平整,无动脉粥样硬化病灶,模型组小鼠血管管径厚薄不均匀,斑块形成明显,血管管壁厚度、AS斑块截面积显著大于其他各组。中药高低剂量组小鼠主动脉各层结构正常,炎性细胞浸润较轻,病变轻,AS斑块较小,病变程度明显轻于模型组。与对照组相比,中药高低剂量组主动脉miR-126、RGS16、CXCL12、CXCR4和VCAM-1 mRNA表达明显优于模型组(P<0.05),中药高剂量组和中药低剂量组间比较差异不明显(P>0.05);与对照组相比,中药高低剂量组主动脉RGS16、CXCL12、CXCR4和V
Objective:To observe the regulation of miRNA-126 and its downstream related signals GRS16, CXCL12, CXCR4 and VCAM-1 in mice aorta and vascular endothelial cells and explore the mechanism of anti-atherosclerosis of traditional Chinese medicine. Methods:In vitro cell experiments, mice vascular endothelial cells were randomly divided into 5 groups, namely, control group, model group, Chinese medicine high-dose drug-containing serum group and traditional Chinese medicine low-dose drug-containing serum group. Different groups’ VEC were treated with different drug-containing serum to observe cell proliferation and apoptosis. qRT-PCR and Western-blot were used to detect pri-miR-126, RGS16, CXCL12, CXCR4, VCAM-1 mRNA and protein in VEC. In vivo experiments,HE staining was used to detect the degree of pathological damage in the aorta of each group. The expressions of pri-miR-126, RGS16, CXCL12, CXCR4, VCAM-1 mRNA and protein in aorta were detected by qRT-PCR and Western-blot. Results:The results of in vitro experiments showed that compared with the control group, the apoptotic rate and proliferation rate of VEC in the high-and low-dose groups of Chinese medicine were significantly better than those in the model group(P<0.05). The proliferation rate of VEC in the high-dose group and the low-dose group of traditional Chinese medicine was not different(P>0.05). Compared with the control group, the expressions of miR-126, RGS16, CXCL12, CXCR4 and VCAM-1 mRNA in the VEC of the high-and low-dose Chinese medicine group was significantly better than that of the model group(P<0.05). There was no significant difference between the low-dose group of traditional Chinese medicine(P>0.05). Compared with the control group, the expressions of RGS16, CXCL12, CXCR4 and VCAM-1 in VEC were significantly better than those in the model group(P<0.05). There was no significant difference between the high-dose group and the low-dose group(P>0.05). The results of in vivo experiments showed that the aortic HE staining of the mice in the control g
作者
秦合伟
李彦杰
任锟
张志鑫
李斯锦
卢永保
QIN Hewei;LI Yanjie;REN Kun;ZHANG Zhixin;LI Sijin;LU Yongbao(Henan Province Hospital of Chinese Medicine,The Second Affiliated Hospital of Henan College,Zhengzhou 450002,Henan,China;Henan University of Chinese Medicine,Zhengzhou 450002,Henan,China)
出处
《中华中医药学刊》
CAS
北大核心
2019年第8期1813-1818,I0007,I0008,共8页
Chinese Archives of Traditional Chinese Medicine
基金
国家自然科学基金项目(81704030)
河南省高等学校重点科研计划项目(18A360008)
河南省科技攻关计划项目(182102311158)
河南省中医药科学研究专项项目(2017ZY2067)
河南省中医临床学科领军人才培育计划项目(2100202)
河南省中医院专项项目(2017YJKT02)
