摘要
目的探讨高良姜素在大鼠肠道的吸收机制。方法采用大鼠在体单向肠灌流吸收实验模型,以质量法校正灌流液体积,采用反相高效液相色谱法测定灌流液中高良姜素浓度,考察不同肠段、药物浓度和P糖蛋白(P-gp)抑制药对高良姜素吸收的影响。结果高良姜素在整个肠段都有吸收,在十二指肠、空肠、回肠和结肠的吸收速率常数Ka分别为(5.12±1.14)×10-2,(2.23±1.02)×10-2,(4.61±0.75)×10-2和(2.68±0.70)×10-2·min-1,同时它在回肠的吸收不受自身浓度和P-gp抑制药盐酸维拉帕米的影响。结论高良姜素在肠道吸收良好,吸收过程以被动扩散为主,不受P-gp外排蛋白的影响。
Objective To investigate the absorption characteristics of galangin in various intestinal segments. Methods Single-pass intestinal perfusion was employed in rats, and the mass quality was used to correct the volume;Galangin in rat intestinal perfusion was determined by HPLC to investigate the effects of intestinal segments, drug concentration and P-glycoprotein （ P-gp） inhibitor on drug’ s absorption. Results Galangin could be absorbed in the whole intestine, and its Ka values in the segments of duodenum, jejunum, ileum and colon were （5. 12±1. 14）·10^-2,（2. 23±1. 02）·10^-2,（4. 61± 0. 75）· 10^-2 and（2. 68 ± 0. 70）·10^-2 ＆#183;min^-1 ,respectively. Meanwhile, the values of the Ka in the segment of ileum were not affected by the drug concentration and P-gp inhibitor. Conclusion The galangin is well absorbed in rats intestinal segments. The absorption procedure is mainly controlled by passive diffusion but unaffected by P-gp efflux protein.
出处
《医药导报》
CAS
2015年第5期612-616,共5页
Herald of Medicine
关键词
高良姜素
肠道吸收
单向肠灌流法
色谱法
高效液相
Galangin
Intestinal absorption
Single-pass intestinal perfusion
Chromatography,high performance liquid
