摘要
目的 比较评价2种登革病毒NS1抗原检测试剂和1种核酸检测试剂在登革热早期诊断中的应用,为登革热实验室检测试剂选择提供依据.方法 选取登革病毒感染发病早期患者血清278份,非登革热患者血清100份,对3种检测试剂进行比较评价.结果 发病5d内患者血清样本,PCR-荧光探针法敏感性为98.9％ （180/182）,ELISA法为97.8％ （178/182）,胶体金法为81.9％（149/182）,发病后6-7d的血清样本,敏感性均有所下降,NS1 ELISA法相对恒定,三种检测试剂均具有较高的特异性.结论 实时荧光RT-PCR法核酸检测试剂在发病早期敏感性最高,ELISA法检测NS1抗原法与核酸检测一致性最强,胶体金法敏感性略差,但更简便快捷,适合基层和现场使用,NS1抗原检测可用于我国登革热病例的监测与检测.
Objective To evaluate the performance of two commercially available dengue nonstructural 1 （NS1） antigen assays and a reverse-transcription polymerase chain reaction （RT-PCR） for the diagnosis of dengue.Methods A panel of dengue positive and negative sera was assembled,and 278 sera from suspected Dengue patients and 100 sera from non-dengue infected people were included to evaluate the diagnostics.Results The analysis showed RT-PCR to be the most sensitive and specific （100%） diagnostic method during the first 5 days of fever,The overall sensitivity of dengue NS1 antigen assays within the same period was 97.8% （178/182）for ELISA and 81.9% （149/182）for NS1 Cassette.And all the 3 diagnostics were highly specificity.Conclusion Real time RT-PCR is the most sensitive method at early stage of the illness; NS1 antigen detection is a convenient method for the diagnosis of dengue fever in a primary healthcare setting,which can be used in the early detection of dengue and hence could play an important role in routine surveillance efforts to control dengue outbreaks in China.
出处
《中华实验和临床病毒学杂志》
CAS
CSCD
2015年第2期154-156,共3页
Chinese Journal of Experimental and Clinical Virology
