摘要
目的分析东莞地区登革热患者实验室检验结果的特点,为临床诊治提供依据和建议。方法选择2019年7—12月东莞康华医院确诊的165例登革热患者作为研究对象。所有患者入院5 d内采用双抗体夹心酶联免疫吸附试验(ELISA)检测登革热病毒NS1抗原,采用胶体金法检测登革热病毒免疫球蛋白(IgM、IgG)抗体;对患者的实验室检验结果进行收集、整理和分析。结果165例登革热患者中,31~40岁年龄段患者占比最多〔32.12%(53/165)〕;登革热病毒NS1抗原阳性检出率高于IgM和IgG抗体检测〔99.39%(164/165)比78.79%(130/165)、20.61%(34/165)〕;所有患者中白细胞计数(WBC)减少129例(78.18%),血小板计数(PLT)减少116例(70.30%),丙氨酸转氨酶(ALT)升高65例(39.39%),天冬氨酸转氨酶(AST)升高124例(75.15%),肌酐(Cr)升高10例(6.06%),肌酸激酶(CK)升高55例(33.33%),乳酸脱氢酶(LDH)升高92例(55.76%),血浆凝血酶原时间(PT)延长24例(14.55%),活化部分凝血活酶时间(APTT)延长97例(58.79%)。结论登革热发病人群以中青年为主;大多数登革热患者WBC、PLT明显降低;超半数患者合并心肌损伤;超半数患者出现内源性凝血系统异常,仅少数患者出现肾损伤;ELISA法检测登革病毒NS1抗原具有敏感性高、特异性好的特点,对疾病早期诊断和疫情的早期控制都具有重要意义,可作为常规方法在基层医疗机构使用。
Objective To analyze the characteristics of laboratory examination results of patients with dengue fever in Dongguan area,in order to provide experimental basis and suggestions for the clinical diagnosis and treatment.Methods The 165 cases of dengue fever diagnosed in Kanghua Hospital of Dongguan City from July to December in 2019 were selected as research objects.Within 5 days of admission,the dengue virus NS1 antigen in all patients was detected by double antibody sandwich enzyme linked immunosorbent assay(ELISA),and dengue virus immunoglobulin(IgM and IgG)antibody was detected by colloidal gold method.Results Among 165 cases of dengue fever,patients at the age of 31-40 years old accounted for the largest proportion[32.12%(53/165)];the positive rate of dengue virus NS1 antigen detection was higher than those of dengue virus IgM and IgG antigen detection[99.39%(164/165)vs.78.79%(130/165),20.61%(34/165)].Among all the patients,there were 129 cases(78.18%)with decreased white blood cell count(WBC),116 cases(70.30%)with decreased platelet count(PLT),65 cases(39.39%)with increased alanine aminotransferase(ALT),124 cases(75.15%)with increased aspartate aminotransferase(AST),10 cases(6.06%)with increased creatinine(Cr),55 cases(33.33%)with increased creatine kinase(CK),92 cases(55.76%)with increased lactate dehydrogenase(LDH),24 cases(14.55%)with increased prothrombin time(PT),and 97 cases(58.79%)with increased activated partial thromboplastin time(APTT).Conclusions Dengue fever mainly occurs in young and middle-aged people;a significant reduction in WBC and PLT occurred in most patients with dengue fever,more than half of them were associated with myocardial damage and more than half of them had endogenous coagulation disorders,only a few patients had kidney injury.ELISA detection of dengue virus NS1 antigen is sensitive and specific.It has a great significance in early diagnosis of disease and early control of epidemic situation,and is suitable for routine use in primary medical institutions.
作者
林佩娜
隋洪
Lin Peina;Sui Hong(Laboratory Center,Kanghua Hospital of Dongguan City,Dongguan 523080,Guangdong,China)
出处
《实用检验医师杂志》
2020年第4期233-235,共3页
Chinese Journal of Clinical Pathologist
关键词
登革热
实验室NS1抗原
结果分析
Dengue fever
Laboratory NS1 antigen
Interpretation of result
