摘要
目的:观察杞椇颗粒剂对雄性大鼠酒精性性功能损伤的预防作用。方法:大鼠随机分为杞椇颗粒剂14g/kg、7g/kg、3.5g/kg组,睾酮4mg/kg组,模型对照组和正常对照组。各给药组动物均灌胃给予56°白酒10ml/kg,同时给予相应药物,正常对照组灌胃等体积蒸馏水,连续45天。各给药组雄鼠均分别与动情期雌鼠1∶1合笼,记录初次发现阴栓时间,阴栓数,交配率。合笼完成后,检测雄鼠血清睾酮水平,计算睾丸、附睾、精囊腺、肾脏等脏器指数。于妊娠第18天处死雌鼠,计算受孕率、胎鼠窝均体重、胚胎吸收率、活胎率、死胎率;将胎鼠做骨骼染色,计算骨骼发育不全率。结果:杞椇颗粒剂14g/kg、7g/kg、3.5g/kg剂量组睾丸指数分别为0.84±0.04、0.88±0.07、0.87±0.04 g/100g均高于模型对照组0.79±0.07 g/100g(P<0.05或P<0.01),附睾指数分别为0.33±0.03、0.34±0.04、0.36±0.32 g/100g均高于模型对照组0.29±0.02 g/100g(P<0.05或P<0.01),精囊腺指数分别为0.
Obgective： To observe preventive effect of Qiju Granulas on sexual function injury induced by alcohol in male rats. Methods： Male SD rats were randomly divided into Qiju Granulas14 g / kg,7 g / kg,3. 5g / kg dose group,testosterone group 4mg / kg,model group and normal con-trol group. All the drug groups were ig with56 °wine and were given the corresponding medicine at the same time. Normal control group were ig with equal volume distilled water,which continued 45 days. Each group of male rats were mated by1： 1with estrous female rats. Record the time when first founding the vaginal plug,vaginal number,mating rate. After mating,content of serum testosterone in serum was tested and index of testis,epididymis,seminal vesicle and kidney were calculated. Female rats were killed at the 18 th of pregnancy,pregnancy rate,average weight of each litter of fetal rat,resorption rate of embryo,rate of live birth and rate of death were calculated; bones of fetal rats were stained and skeletal dysplasia rate was calculated. Results： Testis index of14 g / kg、7g / kg、3. 5g / kg groups of Qiju Granulas were 0. 84 ± 0.04,0. 88 ± 0. 07,0. 87 ± 0. 04 g /100 g,respectively,which were higher than that of model control group 0. 79 ± 0. 07 g /100g（ P〈0. 05,P〈0. 01）; epididymal index were 0. 33 ± 0. 03,0. 34 ± 0. 04,0. 36 ± 0. 32 g /100 g respectively,which were higher than that of model control group 0. 29 ± 0. 02 g /100g（ P〈0. 05,P〈0. 01）; seminal vesicle index were0. 37 ± 0. 07,0. 42 ± 0. 08,0. 42 ± 0. 14 g /100 g respectively,which were higher than that of model control group0. 32 ± 0. 04 g /100g（ P〈0. 05 或 P〈0. 01）; kidney index were 0. 64 ± 0. 05,0. 65 ± 0.06,0. 64 ± 0. 09 g /100 g respectively,which were lower than that of model control group 0. 71 ± 0. 07 g /100g（ P〈0. 05）,the level of serum testosterone were 1. 77 ± 0. 43,1. 81 ± 0. 29,1. 58 ± 0. 23 nmol / L respectively,which were higher than that of model control group 1. 23 ± 0.07 nmol / L（ P〈0. 05,P〈0. 01
出处
《中药药理与临床》
CAS
CSCD
北大核心
2015年第1期192-195,共4页
Pharmacology and Clinics of Chinese Materia Medica
基金
河北省高校重点学科项目资助
关键词
杞椇颗粒剂
大鼠
酒精
性功能损伤
Qiju Granules （杞椇颗粒）
alcohol
sexual function
injury
prevention
