摘要
研究了在绵羊冻精稀释液中添加不同浓度(0、1、2、2.5、3 mmol/L)的谷胱甘肽(GSH)对细管冻精质量及体外受精的影响。结果显示:在绵羊精子的冷冻稀释液中添加1 mmol/L GSH具有最好的效果,可以显著地增加冷冻解冻精子的活力以及精子质膜、顶体膜和DNA的完整性,还可以在一定程度上提高体外受精的卵裂率及囊胚率。
We studied the effects of adding different concentrations （0, 1, 2, 2,5, 3 mmol/L） of glutathione （GSH） into the cryopreserved ovine semen diluent on the quality of cryopreserved ovine semen in experimental extender and the external fertilization. The resttlts showed that adding 1 mmol/L GSH had the best effects, and it could significantly increase the vitality of unfrozen semen as well as the integrity of sperm plasma membrane, acrosome membrane and DNA, and also could enhance the cleavage rate and blastocvst rate of external fertilization to some extent.
出处
《江西农业学报》
CAS
2015年第5期79-83,共5页
Acta Agriculturae Jiangxi
基金
国家转基因重大专项(2013ZX08008-003)
国家自然科学基金项目(U1203381)
新疆维吾尔自治区公益性科研院所基本科研业务经费专项
关键词
绵羊
谷胱甘肽
精液
冷冻
解冻
质量
Sheep
GSH
Semen
Cryopreservation
Unfrozen
Quality
