摘要
以德国鸢尾（Iris germanicaL．）品种“金娃娃”当年新萌发幼芽的茎尖为外植体，进行了组织培养和快速繁殖技术的研究。结果表明：使用0．1％HgCl：对外植体消毒7min的效果最佳；初代培养、继代培养、生根培养最适合的培养基分别是MS＋6-BA1．5mg／L＋IAA0．2mg／L、MS＋6-BA2．0mg／L＋IAA0．5mg／L、1／2MS＋6-BA0．2mg／L＋IAA1．5mg／L；采用含4-5个芽的芽丛进行继代培养的增殖系数最高；试管苗移栽的最佳基质为珍珠岩＋草炭＋园土（1：1：1），移栽成活率达到91．6％。
The stem tips of newly-germinated tender buds of the German iris （Iris germanica L. ） variety ＂Gold boy＂ were taken as explants, and the tissue culture and rapid propagation techniques of iris were studied. The results showed that disinfecting the explants with O. 1% HgC12 for 7 min was the most effective. The optimal media for the primary culture, subculture and rooting culture of iris were MS ＋6-BA 1.5 mg/L＋IAA 0.2 mg/L, MS＋6-BA 2.0 mg/L＋IAA 0.5 mg/L, and 1/2MS＋6-BA 0.2 mg/L＋IAA 1.5 rag/L, respectively. The subculture of bud cluster with 4 - 5 buds obtained the highest propagation coefficient. The optimum sub- strate for the transplanting of iris test-tube plantlets was perlite＋ turf＋ garden soil （ 1：1：1 ）, and the survival rate after being trans- nlanted reached 91.6%.
出处
《江西农业学报》
CAS
2015年第5期25-28,共4页
Acta Agriculturae Jiangxi
关键词
鸢尾
组织培养
快速繁殖
Iris
Tissue culture
Rapid propagation
