摘要
目的：分析子宫腺肌症患者在位与异位内膜组织中r IL-10诱导下HLA-G的表达变化,探讨其临床意义。方法：选取该科2013年3~10月收治的子宫腺肌症患者中符合研究需求的在位子宫内膜组织与异位子宫内膜组织各52例,选取同时期收治的子宫腺肌症以外的其他子宫良性疾病患者子宫内膜52例设为对照组,进行r IL-10诱导处理后,运用免疫显色法测定HLA-G的阳性表达及其程度,通过内膜组织染色的深浅程度和存在染色反应的细胞数占总细胞数的比例进行综合评分。结果：研究组在位与异位内膜的HLA-G阳性率相对对照组均有较大变化,差异具有统计学意义（P〈0.05）;而研究组在位内膜和研究组异位内膜之间及增生期和分泌期之间的HLA-G阳性率差异无统计学意义（P〉0.05）。结论：检测r IL-10诱导下HLA-G的异常表达可提示发生子宫腺肌症的高危人群,建议临床推广使用。
Objective： To analyze the change of expression of human leukocyte antigen-G （HLA-G） induced by rIL-10 in eutop- ic and ectopic endometrium of patients with adenomyosis, explore the clinical significance. Methods： Fifty＇two specimens of eutopic endo- metrium and fifty-two specimens of ectopic endometrium were selected from the department among the patients with adenomyosis meeting the inclusion criteria from March to October in 2013 ; fifty-two specimens of normal endometrium were selected as control group from the patients with benign uterine lesions excluding adenomyosis during the same period, after induction with rIL-10, immune colorimetric method was used to determine positive expression and degree of HLA-G. Comprehensive scoring was performed according to the staining degree and the percentage of cells with staining reaction in total cells. Results ： There was statistically significant difference in the positive rate of HLA-G between eutopic and ectopic endometrium in research group and control group （ P〈0. 05 ） ; but there was no statistically significant difference in the positive rate of HLA-G between eutopic endometrium arid ectopic endometrium in research group, as well as between proliferative phase and secretory phase （P〉0.05） . Conclusion： Abnormal expression of HLA-G induced by rIL-10 may indicate high risk population of adenomyosis, the detection method is worthy of clinical promotion and application.
出处
《中国妇幼保健》
CAS
2015年第14期2161-2163,共3页
Maternal and Child Health Care of China
基金
山东省自然科学基金资助项目〔ZR2013HM060〕
