摘要
采用高效液相色谱-蒸发光散射检测法测定了盐酸氨基葡萄糖胶囊的含量.使用氨基色谱柱（250 mm×4.6 mm,5μm）,流动相组成为乙腈：水=（7：3）,流速为1.0 mL/min,在盐酸氨基葡萄糖检测含量浓度范围为0.2-1.2 mg/mL时与峰面积呈线性关系,R^2为0.9999,平均加样回收率为99.86%,RSD为1.37%.该法简单,准确,无需衍生反应,精密度和重现性较好,是盐酸氨基葡萄糖胶囊含量测定的有效方法.
To establish a high performance liquid chromatography-scattering detector（ HPLC-ELSD） method for the Glucosamine Hydrochloride in Glucosamine Hydrochloride Capsules. The HPLC-ELSD was performed on a NH2column （250mm ×4. 6mm,5μm）. The mobile phase was a mixture of acetonitrile and water （7：3）. The flow was 1. 0mL/min. Within the range of 0. 2-1. 2mg/mL, there was a good linear relation between the concentration and peak area of glucosamine hydrochloride （R^2 =0. 9999）. The average recovery was 99. 86%（ RSD=1. 37%） . This method is simple, accurate, specific, reproducible, and it has not derivation. It can be applied to determine the content of Glucosamine Hydrochloride in Glucosamine Hydrochloride Capsules.
出处
《淮阴师范学院学报（自然科学版）》
CAS
2015年第1期48-51,共4页
Journal of Huaiyin Teachers College;Natural Science Edition
