摘要
为拓宽花生的耐盐基因资源,前期以平阳霉素为诱变剂进行了花生离体诱变,并在培养基中添加Na Cl进行定向筛选,获得了一批Na Cl耐性苗及其后代。对Na Cl耐性植株M3个体的荚果产量进行了测定,结果表明,有5个原始再生植株产生了3个以上大于60 g荚果重的M3单株。用近红外仪对M4种子的各品质性状进行测定,结果发现,其在蛋白质、油酸、亚油酸及脂肪含量发生了广泛变异,有7份变异材料的蛋白质含量超过30%,11份变异材料的脂肪含量超过55%。结合M4种子在0.7%盐溶液中的发芽情况,筛选出几个综合了高产、高油、耐盐多个优良性状的个体。证明PYM离体诱变、Na Cl定向筛选与近红外技术的有效结合,在花生中是一条新的有效的育种途径。
To expand the salt-tolerant gene resources of peanut ,we conducted in vitro mutagenesis with pingy-angmycin（PYM）as the mutagen and directed screening on a medium containing NaCl in the previous experiment .In this study ,pods of M 3 individual plants from those original NaCl-tolerant plants were collected and weighted after harvest and sun drying and the results indicated that 5 original HYP-tolerant,regenerated plants produced≥3 off-spring with over 60 g/pods per plant .M4 seeds were tested for quality by Near Infrared Spectroscopy ,and the results indicated that there was substantial variation in the protein content ,oleic acid,linoleic acid content,and oil content, including seven with higher protein content （〉30%） and 11 individuals with higher oil content （〉55%） .Accord-ing to the results of pod weight per plant and quality traits combined with a germination test with a 0 .7%NaCl so-lution of M4 seeds,we screened a few individuals with high yield ,oil content and salt tolerance .We concluded that the use of PYM-based in vitro mutagenesis in combination with directed screening with NaCl and Near Infrared Spectroscopy technology is a new effective pathway for breeding of peanut .
出处
《华北农学报》
CSCD
北大核心
2015年第1期202-206,共5页
Acta Agriculturae Boreali-Sinica
基金
国家自然科学基金项目(31301356)
山东省科技发展计划项目(2014GNC110002)
山东省农业良种工程项目
青岛农业大学大学生科技创新项目
关键词
花生
平阳霉素
定向筛选
近红外技术
品质
Peanut
Pingyangmycin（PYM）；Directed screening
Near infrared spectroscopy technology
Quality
