摘要
通过考马斯亮蓝法测定在不同光照和孵育时间下对已知不同浓度的牛血清蛋白的影响.结果显示,在0～100μg/ml蛋白质浓度标准曲线测定范围内,黑暗环境测定蛋白质误差率最小.在黑暗和弱光条件下高浓度蛋白质组（50～75μg/ml）误差率最低显著低于低浓度蛋白质组（5～25μg/ml）.在黑暗和弱光条件下浓度在50～75μg/ml范围内的蛋白质与染料孵育30 min以内时误差率较低且较稳定.因此,采用考马斯亮蓝法测定蛋白质浓度时,环境光照、待测样品浓度以及染料与蛋白质孵育时间等是决定实验结果准确性的重要因子.
This study aimed to investigate the effects of light effect on determination of protein concentration with Coomassie Brilliant Blue method,thus proving advice and guidance for accurate determination of protein concentration. With Coomassie Brilliant Blue method,the concentrations of different bovine serum albumin samples were determined under different light and incubation time. The results showed as follow：when the determination range of protein concentration was 0 ～100 μg/ml,the deviation of protein concentration is the lowest under darkness. Under darkness and room light,the higher range of protein concentration（ 50 ～75 μg/ml ）was lower deviation than the lowerer range of protein concentration（5～25 μg/ml）. Meanwhile,Under darkness and room light,Only whitin incubation 30 min between the 50～75μg/ml protein concentration and Coomassie brilliant blue showed the lowest and the moststable deviation. Therefore,light,sample concentration and incubation time were important factors affecting the accuracy of experimental results in determination of protein concentration with Coomassie Brilliant Blue method.
出处
《吉林师范大学学报（自然科学版）》
2015年第2期112-116,共5页
Journal of Jilin Normal University:Natural Science Edition
基金
吉林省自然科学基金项目基金资助(201115221)
关键词
考马斯亮蓝法
蛋白质
光照
浓度
孵育时间
Coomassie brilliant blue method
protein
light
concentration
incubation time
