摘要
在水温23．5～28．5℃下，将体质量（6．76±0．1）g的中华绒螯蟹随机分为6组，置于24个100 cm ×50 cm ×50 cm的玻璃缸中，每组4个平行，每个平行30尾中华绒螯蟹，投喂含有0（对照组）、0．1％、0．5％、1．0％、1．5％、2．0％复方中草药的饲料60d。试验结果表明，除0．1％组与对照组差异不显著（P＞0．05）外，其他各试验组中华绒螯蟹的质量增加率和特定生长率均显著高于对照组（P＜0．05）。1．0％组中华绒螯蟹血细胞吞噬率和吞噬指数最高（P＜0．05）；血清和肝胰脏组织中超氧化物歧化酶、过氧化物歧化酶、过氧化氢酶的活性最高，与对照组差异显著（P＜0．05）。血清中酸性磷酸酶活性，除0．1％和2．0％组（P＞0．05）外，其他各处理组酸性磷酸酶活性显著升高（P＜0．05）。0．5％、1．0％和1．5％组中华绒螯蟹的碱性磷酸酶活性与对照组差异显著（P＜0．05），其他各组与对照组差异不显著（P＞0．05）。血清和肝胰脏组织中溶菌酶活性均随饲料复方中草药添加量的增加先升后降，显著高于对照组（P＜0．05）。肝胰脏中丙二醛的含量随着复方中草药添加量的增加而显著下降。除0．1％组外，其他组的中华绒螯蟹死亡率显著降低（P＜0．05），免疫保护率显著性升高（P＜0．05）。在基础饲料中添加1．0％的复方中草药，中华绒螯蟹生长、非特异性免疫及抗病力较强。
In a feeding trial ,the effects of a Chinese herbal compound on grow th performance ,non‐specific immune response and disease resistance were investigated in Chinese mitten‐handed crab Eriocheir sinensis with body weight of （6 .76 ± 0 .1） g at water temperature of 23 .5—28 .5 ℃ .The crab was reared in 24 tanks of each 100 cm × 50 cm × 50 cm at a stocking density of 30 individuals per tank and fed diets containing Chinese herbal compound at a rate of 0% （control group ） ,0 .1% ,0 .5% ,1 .0% ,1 .5% and 2 .0% for 60 days with four replicates per group .Except 0 .1% group ,there were significantly higher weight gain and specific growth rate in the crab in the treatment groups than those in the control group （P〈0 .05） .In the 1 .0% group ,the crab had the maximal phagocytosis percentage and phagocytic index in the blood cells and activities of superoxide dismutase （SOD） ,peroxidase （POD） and catalase in the serum and hepatopancreas ,significantly higher than those in the other groups （P〈0 .05） .The activities of the serum acid phosphatase （ACP） were found to be significantly higher among the treatment groups except in 0 .1% and 2 .0% groups .There were significant differences in activities of serum alkaline phosphatase （AKP） between the 0 .5% ,1 .0% and 1 .5% groups and the control group （P〈0 .05） .The activities of serum and hepatopancreas lysozymes （LSZ ） were first found to be increased and then decreased with increase in dietary Chinese herbal compound ,significantly higher than those in the control group （ P〈0 .05） . Hepatopancreas malondialdehyde （MDA ） content was decreased with increase in the dietary Chinese herbal compound .The 15 d challenge with Aeromonas hydrophila revealed that the mortality rates were significantly decreased in the treatment groups than that in the control group except in 0 .1%group （P〈0 .05） ,and the immune protective rate was increased with the elevated dietary Chinese herbal compound .In conclusion ,the diet con
出处
《水产科学》
CAS
CSCD
北大核心
2015年第4期201-207,共7页
Fisheries Science
基金
江西省水利厅重大公益性研究项目(KT201202)
关键词
复方中草药
中华绒螯蟹
生长性能
非特异性免疫
抗病力
Chinese herbal compound
Eriocheir sinensis
grow th performance
non-specific immune
disease resistance
