摘要
目的观察黄芪多糖配合心理护理对直肠癌术后患者细胞免疫及血清炎性因子表达的影响。方法选取160例直肠癌患者随机分为常规护理组、心理护理组、黄芪多糖组和黄芪多糖配合心理护理组(联合组)。采用流式细胞术对患者外周血T细胞亚群进行检测。应用实时定量PCR法和ELISA法检测各组患者血清炎性细胞因子mRNA表达水平和蛋白浓度。结果与常规护理组相比,黄芪多糖组患者手术后3项免疫指标(CD4+、CD4+/CD8+及NK)升高,血清中TNF-α的蛋白含量和mRNA表达水平明显下降;与常规护理组相比,心理护理组患者治疗后NK升高,TNF-α、IL-6和IL-8的蛋白含量和mRNA表达水平明显下降;与常规护理组相比,联合组患者治疗后细胞免疫4项指标(CD3+、CD4+、CD4+/CD8+及NK)升高,CD8+下降,血清中TNF-α、IL-6、IL-8和IL-12的蛋白含量和mRNA表达水平明显下降;与黄芪多糖组相比,联合组患者术后细胞免疫2项指标(CD3+和NK)升高,TNF-α和IL-12的蛋白含量和mRNA表达水平明显降低。结论黄芪多糖配合心理护理不仅调节细胞免疫功能,还能降低直肠癌患者血清中炎性因子的表达水平,从而增强直肠癌患者免疫力。
Objective To investigate the effect of astragalus polysaccharide（ APS） combined with psychological nursing on cellular immunity and expression of proinflammatory cytokines in patients after rectal cancer operation. Methods 160 rectal cancer patients were randomly divided into routine nursing（ RN） group,APS group,psychological nursing（ PN） group and combined group（ APS combined with psychological nursing）,40 patients in each group. The T cell subsets after operation of 160 patients were detected by flow cytometry. ELISA was used to detect the contents of serum proinflammatory cytokines protein; Real-time PCR was used to detect the expression levels of cytokines mRNA. Results Compared with RN group,the CD4＋,CD4＋/ CD8＋and NK indexes in APS group increased significantly,the contents and expression levels of TNF-α protein and mRNA in APS group decreased significantly（ P〈0. 05）; the NK in PN group increased significantly（ P〈0. 05）; the contents and expression levels of TNF-α,IL-6 and IL-8 proteins and mRNA in PN group decreased significantly（ P〈0. 05）,the CD3＋,CD4＋,CD4＋/ CD8＋and NK in combined group increased significantly,the CD8＋indexes in combined group decreased significantly,the contents and expression levels of TNF-α,IL-6 and IL-8 proteins and mRNA in combined group decreased significantly,there were significant differences（ P〈0. 05）. Compared with APS group,the CD3＋and NK in combined group increased significantly,the contents and expression levels of TNF-α and IL-12 proteins and mRNA in combined group decreased significantly. Conclusion APS combined with psychological nursing can not only regulate the immuniological function,but also reduce the expression levels of inflammatory cytokines in serum of rectal cancer patients,so as to improve the immunity of patients.
出处
《实用药物与临床》
CAS
2015年第4期425-428,共4页
Practical Pharmacy and Clinical Remedies
基金
沈阳市科学技术项目计划(F13-318-1-69)
关键词
黄芪多糖
心理护理
直肠癌
细胞因子
Astragalus polysaccharide
Psychological nursing
Rectal cancer
Cytokines
