摘要
目的:观察病毒性心肌炎(VMC)小鼠急性期不同时点白细胞介素21(IL-21)的表达及其与抗腺嘌呤核苷酸转位子(adenine nucleotide translocator,ANT)抗体的相关性,探究其在VMC发病中的作用及意义。方法:BALB/c小鼠腹腔注射柯萨奇病毒B3(CVB3)建立VMC模型,腹腔注射等量PBS的BALB/c小鼠作为对照组,在注射的第0、1、2、3、4和6周,应用荧光定量逆转录-聚合酶链反应(qRT-PCR)检测小鼠心肌组织中IL-21mRNA的表达、酶联免疫双夹心抗体法(ELISA)检测血清IL-21蛋白及抗ANT抗体的表达。结果:与对照组比较,VMC小鼠心肌组织中IL-21mRNA及血清中IL-21自1周开始高表达,2周时达峰值,并维持高表达至6周,各时点表达量均高于相应时点的对照组(P<0.05)。血清中抗ANT抗体在第1周时开始升高,2周时达峰值,维持高水平表达至第6周,除0周时点外,VMC小鼠与对照组小鼠比较差异均有统计学意义(P<0.05),Pearson相关分析显示,IL-21与抗ANT抗体存在相关性(r=0.88,P<0.01)。结论:IL-21在VMC小鼠心肌组织及外周血中高表达,并与血清抗ANT抗体呈正相关,提示IL-21参与VMC的发病过程。
Objective：To explore the expression of IL-21 and the relationship with adenine nucleotide translocator antibody,（ANT antibody） in different courses of viral myocarditis（ VMC） mice,and to explore the role and significance of IL-21 in VMC. Methods： To induced VMC mice model,BALB / c male mice were peritoneally injected（ IP） with Coxsackievirus B3,mice peritoneally injected with PBS were taken as the controls. On 0,1,2,3,4 and 6 w after IP,the level of IL-21 mRNA in mice myocardic tissue was determined by real-time RT-PCR. The expression of IL-21 protein and ANT antibody in serum was evaluated by EILSA. Results：Comparing with the controls,the expression of IL-21 mRNA and protein increased from 1st week after IP,reaching the peak on the 2nd week,and maintaining a higher trend until 6th week. All the results at each time except that of 0 week were higher than those of controls（P〈0. 05）. The levels of ANT antibody were also increased from 1st to 6th week,reaching the peak on the 2nd week all the results at each time except that of 0 week were higher than those of controls（ P〈0. 05）. Pearson analysis showed that there was relationship between IL-21 and ANT antibody（ r = 0. 88,P〈0. 01）. Conclusion： The datas show that local significantly increased levels of IL-21 in myocardium and serum,and have positive relationship with the level of adenine nucleotide translocator antibody,which indicates that IL-21 may play a role in the pathogenesis of mice virus myocarditis.
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
2015年第4期453-455,461,共4页
Chinese Journal of Immunology
基金
国家自然科学基金项目(No.81260046)
