摘要
目的：探讨寿胎丸对实验性流产大鼠的安胎作用及作用机制。方法：每只大鼠尾静脉注射细菌脂多糖（LPS）1.0μg/ml制备流产模型,正常怀孕大鼠每只尾静脉注射生理盐水1.0 ml。取怀孕7天的Wistar大鼠60只随机分为对照组、流产模型组和寿胎丸安胎组各20只,对照组与流产模型组分别于3-9天口服生理盐水2 ml/d,寿胎丸安胎组怀孕3-9天口服寿胎丸2 ml（10 mg）/d,在孕后第10天麻醉处死各组大鼠观察流产率;采用实时荧光定量法和酶联免疫法检测实验性流产大鼠外周血中IL-1b、IL-8和IL-10的mRNA水平及蛋白含量。结果：与对照组相比,寿胎丸安胎组大鼠外周血中IL-1b、IL-8的mRNA水平及蛋白含量略升高和IL-10的mRNA水平及蛋白含量略降低,差异均无统计学意义（P〉0.05）;流产模型组大鼠外周血中IL-1b、IL-8的mRNA水平及蛋白含量明显升高和IL-10的mRNA水平及蛋白含量明显降低,差异均有统计学意义（P〈0.01）。结论：寿胎丸可通过下调IL-1b、IL-8水平和上调IL-10水平使妊娠维持正常状态,具有一定的安胎作用。
Objective： To explore fetus protection effect of Shoutai pills on experimental abortion rats and the mechanisnL Meth- ods： Rat model of abortion was established by intravenous injection of 1. 0 μg/ml lipopolysaeeharide （LPS） into caudal vein, the normal pregnant rats were treated with intravenous injection of 1. 0 ml normal saline into caudal vein. Sixty pregnant Wistar rats for seven days were selected and randomly divided into control group, abortion model group, and fetus protection group, 20 rats in each group; the rats in control group and abortion model group were treated with oral administration of normal saline from the third day to the ninth day, 2 ml per day ; the rats in fetus protection group were treated with oral administration of Shoutal pills from the third day to the ninth day, 2 ml （I0 rag） per day, the rats were put to death on the tenth day after pregnancy, the abortion rates were observed; real-time fluorescent quantitative method ELISA were used to detect the levels of IL-1b, IL-8, and IL-10 mRNA and proteins in peripheral blood of experimental abortion rats. Re- sults： Compared with control group, IL-1b and IL-8 mRNA and proteins levels in peripheral blood of fetus protection group increased slightly, IL-10 mRNA and protein levels decreased slightly, there was no statistically significant difference （P〉0. 05 ） ; IL-1b and IL-8 mRNA and proteins levels in peripheral blood of abortion model group increased significantly, IL-10 mRNA and protein levels decreased sig-nificantly, there were statistically significant differences （P〈0.01 ） . Conclusion： Shoutai pills can maintain normal state of pregnancy by downregulating the levels of IL-8 and IL-lb and upregulating the level of IL-10 in rats, which has a certain fetus protection effect.
出处
《中国妇幼保健》
CAS
2015年第13期2092-2094,共3页
Maternal and Child Health Care of China
基金
辽宁省自然科学基金项目〔2013020198〕
关键词
寿胎丸
实验性流产
白细胞介素
Shoutai pill
Experimental abortion
Interleukin
