摘要
15名青年男子田径运动员在功率自行车上进行一次力竭运动,于运动前1d（Baseline）、运动前（Pre-Ex）、运动后即刻（Post-Ex）、运动后24h（Post-24h）、48h（Post-48h）和72h（Post-72h）分别测定肌肉酸痛强度,血浆、尿和淋巴细胞中8-羟基脱氧鸟苷（8-OHdG）含量以及血浆中肌酸激酶（CK）活性和丙二醛（MDA）含量,探讨8-OHdG作为运动机能监控标志物的可行性.结果显示,肌肉酸痛强度在运动后即刻明显升高（P〈0.01）,24h达峰值（P〈0.01）,72h恢复至正常水平;血浆CK活性在运动后24h开始升高（P〈0.01）并持续至72h（P〈0.01）;血浆MDA含量和淋巴细胞8-OHdG含量均在运动后即刻显著升高（P〈0.01）,24h恢复至安静水平（P〉0.05）;血浆和尿液中8-OHdG含量在各时间点均无显著性变化（P〉0.05）.血浆和尿液中8-OHdG含量在Baseline、Pre-Ex的两次测试间均无显著性差异,日间变异系数（CV）分别为72.8%（95%CI：57.2%~99.9%）和87.8%（95%CI：66.1%~130.5%）.ELISA法测定8-OHdG的批内CV为2.25%~4.67%,批间CV为5.25%~7.12%.以上结果表明,虽然力竭运动可造成氧化应激、淋巴细胞DNA损伤和运动性肌肉损伤,但由于血浆和尿液中8-OHdG含量存在显著的日间生物节律变异,因此可能不是运动机能监控（DNA氧化损伤及过度疲劳）的敏感标志物.
Fifteen male adolescent track and field athletes performed an exhausted exercise on cycle ergometer.Muscular soreness ratings,8-OHdG of plamsa,urine and lymphocyte as well as plasma CK and MDA were measured at Baseline（1d before exercise）,Pre-Ex,Post-Ex,Post-24 h,48 h and 72 h to invesigate the feasibility of body fluid 8-hydroxydeoxyguanosine as marker of exercise function monitoring.The results showed that,muscular soreness ratings increased at Post-Ex（P〈0.01）,peaked at Post-24h（P〈0.01）and recovered at Post-72h;plasma CK raised at Post-24h（P〈0.01）and lasted for 72h（P〈0.01）;both plasma MDA and lymphocyte 8-OHdG elevated at Post-Ex（P〈0.01）and regained at Post-24h;there were no significant changes of plasma and urinary 8-OHdG at all time-point（P〉0.05）.No significant difference was found in plasma and urinary 8-OHdG at Baseline and Pre-Ex,while day-to-day coefficients of variation（CV）were 72.8%（95%CI：57.2% ~99.9%）and 87.8%（95%CI：66.1% ~130.5%）,respectively.Intraassay CV and interassay CV of 8-OHdG measured by the ELISA methods were 2.25% ~4.67% and 5.25% ~7.12%,respectively.Data above indicated that plasma and urine 8-OHdG may not be a sensitive monitoring marker of high intensity exercise inducing DNA oxidative damage and overfatigue due to significant day-to-day biological rhythm,although acute exhausted exercise may induce oxidative stress,lymphocyte DNA damage and muscle damage.
出处
《河南大学学报（自然科学版）》
CAS
2015年第2期192-196,共5页
Journal of Henan University:Natural Science
基金
河南省科技攻关项目(142102310060)
