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两种用FITC标记红桂木凝集素方法的比较 被引量:1

Comparison study of two methods labeling ALL with FITC
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摘要 目的:红桂木凝集素(Artocarpus lingnanensis lectin,ALL)与异硫氰酸荧光素(FITC)偶联方法的探究。方法:分别采用透析法和固化法使FITC与红桂木凝集素偶联,检测偶联物的F/P值。结果:固相化法的F/P值达1.02,其它方法 F/P值约为0.758。结论:采用固化法使FITC标记红桂木凝集素,实验重复性较好,方法稳定可靠,且可获得较高偶联率的红桂木凝集素偶联异硫氰酸荧光素(ALL-FITC)利于后续实验研究。 Objective: To investigate the method of labeling artocarpus lingnanensis lectin(ALL) with FITC. Methods. It was cal- culated separately the ratio of FITC to artocarpus tingnanensis lectin(ALL) of the FITC-labeled ALL made by dialysis method and solidification method. Results: The F/P ration of FITC-labeled ALL made by solidification method was 1.02, the F/P ration of FITC-labeled ALL made by dialysis was 0. 758. Conclusions: Both of the two methods of labeling ALL with FITC were successful, the solidification method is better than dialysis method.
出处 《四川生理科学杂志》 2015年第1期1-3,共3页 Sichuan Journal of Physiological Sciences
基金 国家自然科学基金(编号:81160366) 广西自然科学基金(桂科攻10124001A-23)
关键词 FITC 凝集素 透析 浓缩 FITC Lectin Dialysis Solidification
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  • 1Poland CR, Chen YF, Rinderle SJ, et al. Use of the lectin from Amaranthus caudatus as a histochemical probe of proliferating colon ic epithelial cells[J]. Cancer Res, 1991, 5l(2) :657--665. 被引量:1
  • 2Sams JS, Lynch HT, Burr RW, et al. Abnormelition of lectin histo- chemistry in familial polyposis eoli and hereditary nonpolytxosis colo- rectal caneer[J]. Cancer, 1990, 66(3) :502-508. 被引量:1
  • 3Lotan R, Ito H, yasui W, et al. Expression of a 31-kda lac- tosidebinding lectin in normal human gastric mucosa and in primary and metastatic gastric carcinomas[J]. Int J Cancer, 1994, 56 (1) : 474--480. 被引量:1
  • 4黄忠发,张淑真,蓝正文,吴坤烈,陈淑颖,林意文,郭士民.利用凝集素修饰的二氧化硅玻璃球去除胶原蛋白中的内毒素[J].分析化学,2013,41(10):1482-1486. 被引量:1
  • 5张惟杰.栅复合物生化研究技术[M].杭州:浙江大学出版社,1999. 被引量:1
  • 6Goldstein IJ, Hughes RC, Monsigny T, et al. What should be called a leetin[J]. Nature, 1980, 285;66--66. 被引量:1
  • 7鲍锦库.植物凝集素的功能[J].生命科学,2011,23(6):533-540. 被引量:33
  • 8蔡茜茜,李巧玲,刘舒云,汪少芸.植物凝集素研究与展望[J].食品科学技术学报,2013,31(6):51-57. 被引量:12
  • 9Strauchen JA. I.ectin receptors as markers of lymphoid cells. II. Reed-Sternberg cells share lectin-binding properties of monocyte macrophages[J]. Am J Pathol, 1984, 116(3):370--376. 被引量:1
  • 10Kellokumpu IH. Differences in lectin reactivities of cellular glyeo- conjugates between primary human coloreetal carcinomas and their metastases[J]. Cancer Res, 1986, 46(9):4620--4625. 被引量:1

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