摘要
林木分子指纹图谱是优良林木育种资源的管理、利用和产权保护的重要参考资料。本研究基于SSR和SRAP分子标记技术,对福建省漳平五一国有林场马尾松种子园131个亲本无性系进行了指纹图谱构建。从近缘种的168个SSR标记中筛选出15个多态性高、重复性好、条带清晰的SSR标记,在131个无性系中共检测到55个等位基因,平均每个标记3.67个等位基因,平均有效等位基因数为2.04。其中10个SSR位点组合可有效区分116个无性系,增加剩余5个SSR位点可区分的无性系不再增加,但可将剩下无性系（15个）区分为6个组,每个组含2~5个无性系,在此基础上利用筛选出的2对SRAP标记组合可将15个无性系完全区分,最终构建了种子园131个无性系的DNA指纹图谱。基于个体间的遗传距离可将131个无性系分为8类,为后续无性系间的组配和再选育提供参考。
Molecular fingerprinting is an important reference for management and utilization of forest breeding material. In this study, the fingerprint of 131 clones in Pinus massoniana seed orchard was constructed based on SSR and SRAP molecular markers. The 15 SSR markers with high polymorphic, good repeatable, and clear bands were selected among 168 SSR markers which derived from other conifers. A relatively high level of genetic diversity was detected in 131 clones with 15 SSR loci. Average number of alleles（A） and effective number of alleles（Ne） were 3.67 and 2.04. The 116 clones could be effectively distinguished by 10 SSR loci combination, but the 15 clones remained could be only subdivided into six groups（each containing 2~5 clones） with 15 SSR. However the15 clones could be completely differentiated by two pairs of SRAP marker combination. Eventually, the DNA fingerprinting of all 131 clones was constructed with 10 SSR and 2 SRAP. The 131 clones were divided eight clusters based on the genetic distance between individuals, it will be helpful for further breeding work.
出处
《分子植物育种》
CAS
CSCD
北大核心
2015年第3期646-652,共7页
Molecular Plant Breeding
基金
福建省林木种苗科技攻关四期项目
江苏高校优势学科项目(PAPD)共同资助
