摘要
目的探究血管内皮生长因子(VEGF)基因多态性与动脉瘤性蛛网膜下腔出血(a SAH)的相关性。方法采用病例对照的研究方法,实验组为158例a SAH患者,均行颅脑CT检查证实为a SAH,并经脑血管造影证实是否存在颅内动脉瘤;对照组为146名健康成年人。取两组外周血,提取基因组DNA。检测VEGF2578C/A的基因型及等位基因分布频率。比较各位点基因型频率的观察值与预期值并进行卡方检验和Hardy-Weinberg平衡分析,以及VEGF基因型与a SAH的相关性。结果 1对照组VEGF的2个多态位点的基因型频率分布符合Hardy-Weinberg平衡(P>0.05)。2VEGF2578C/A基因型及等位基因分布,CC+CA基因型频率,实验组显著高于对照组(P<0.05);实验组的C等位基因频率明显高于对照组,两组比较差异有统计学意义(χ2=4.256,P=0.037)。结论 VEGF基因启动子区-2578C/A多态性可能与aSAH有关。
Aim To investigate the correlation between vascular endothelial growth factor （VEGF） gene- polymorphisms and aneurismal subarachnoid hemorrhage （aSAH）. Methods Case-control study method was used in this study, the experimental group was composed of 158 cases of aSAH patients and the control group was composed of 146 healthy adults. Patients in experimental group were all conifrmed to have subarachnoid hemorrhage by CT and have aneurysm by DSA. Peripheral blood collected from everyone in both of the groups was used to extract genome DNA in order to detect the genotype and frequency of allele of VEGF. The observed value and expected value of genotype frequency at special locus were compared, and Hardy-Weinberg balance analysis viaχ2 test were done. The correlation of genetype of VEGF and morbidity of aSAH and correlation of genotype of VEGF and subarachnoid hemorrhage were discussed. Results Distribution of VEGF genotype at the two polymorphic sites conformed to H-W balance analysis （P〉0.05） in both the control and the experimental group. Frequency of CC and CA in the experimental group was higher than the control group. Conclusion Polymorphism of VEGF-2578C/A may be associated with aneurysmal subarachnoid hemorrhage.
出处
《中国临床神经科学》
2015年第2期151-155,共5页
Chinese Journal of Clinical Neurosciences
关键词
血管内皮生长因子
基因多态性
动脉瘤
蛛网膜下腔出血
vascular endothelial growth factor
gene polymorphism
intracranial aneurysm
subarachnoid hemorrhage
