摘要
目的探讨芪冬活血饮对急性肺损伤(acute lung injury,ALI)大鼠炎性因子及Toll样受体(Tolllike receptor,TLR)4 mRNA的影响。方法将50只健康雄性SD大鼠,按体重分层随机分为空白对照组、脂多糖(Lipopolysaccharide,LPS)模型组及芪冬活血饮低、中、高剂量组,每组10只。芪冬活血饮低、中、高剂量组分别于造模前24、12 h及造模后12 h给予4、8、16 m L/kg芪冬活血饮灌胃,空白对照组、LPS模型组给予生理盐水灌胃。采用气管内滴入LPS制备大鼠模型,造模24 h后处死动物,制备肺泡灌洗液,采用ELISA法测定TNF-α、IL-1β及IL-10水平;RT-PCR测定肺组织TLR4 mRNA表达。结果与空白对照组比较,LPS模型组TNF-α、IL-1β及IL-10含量均增高(P<0.01),TLR4 mRNA表达亦增高(P<0.01)。与LPS模型组比较,芪冬活血饮高、中剂量组TNF-α、IL-1β含量降低(P<0.05,P<0.01),IL-10含量增高(P<0.01),且TLR4mRNA表达降低(P<0.01)。与芪冬活血饮高剂量组比较,芪冬活血饮中、低剂量组TNF-α、IL-1β含量增高(P<0.05),低剂量组IL-10水平降低(P<0.05),低剂量组TLR4 mRNA表达亦增高(P<0.05)。与芪冬活血饮中剂量组比较,芪冬活血饮低剂量组IL-10水平降低(P<0.05),而TLR4 mRNA表达增高(P<0.05)。结论芪冬活血饮对LPS导致的大鼠ALI有保护作用,其机制与抑制TLR4 mRNA表达,进而导致促炎细胞因子TNF-α、IL-1β下降、抗炎细胞因子IL-10升高,纠正炎症失衡有关。
Objective To explore the effect of Qidong Huoxue Decoction （QHD） on inflammatory factors and Toll-like receptor （TLR4） mRNA expressions in acute lung injury （ALl） rats. Methods Total- ly 50 healthy male SD rats were randomly divided into the blank control group, the lipopolysaccharide （LPS） model group, low, middle, high dose QHD groups according to body weight, 10 rats in each group. Rats in low, middle, high dose QHD groups were intragastrically administered with QHD at 4, 8, and 16 mL/kg 24, 12 h before modeling and 12 h after modeling, respectively. Normal saline was intragastri- cally administered to rats in the blank control group and the LPS model group. An ALl rat model was es- tablished using intratracheal instillation of LPS. Rats were killed after 24-h modeling. Then the bronchoal- veolar lavage fluid was prepared. Contents of TNF-α, IL-1β, and L-10 were detected using ELISA. TLR4 mRNA expressions were determined byreal time PCR. Results Compared with the blank control group, contents of TNF-α, IL-1β, and IL-10 increased （P 〈0.01 ）, TLR4 mRNA expressions also increased in the LPS model group （all P 〈0.01 ）. Compared with the LPS model group, contents of TNF-α and IL-1β de-creased （P 〈0.05, P 〈0.01 ）, IL-10 levels increased （P 〈0.01 ）, TLR4 mRNA expressions were also re- duced （P〈0.01）, in high and middle dose QHD groups. Compared with the high dose QHD group, con- tents of TNF-α and IL-1β increased in middle and low dose QHD groups （P 〈 0.05） ; IL-10 levels decreased （P 〈0.05）in the low dose QHD group（P 〈0.05）, TLR4 mRNA expressions also increased in the low dose QHD group （P 〈0.05）. Compared with the middle dose QHD group, IL-10 levels was reduced, but TLR4 mRNA expressions increased in the low dose QHD group （P 〈0.05）. Conclusions QHD had the protective effect on LPS induced ALl rats. Its mechanism might be associated with inhibiting TLR4 mR- NA expressions, leading to decreased pro-inflammatory cytokines such as TN
出处
《中国中西医结合杂志》
CAS
CSCD
北大核心
2015年第4期438-442,共5页
Chinese Journal of Integrated Traditional and Western Medicine
基金
浙江省自然科学基金资助项目(No.LQ12H29003)
