摘要
背景:目前临床上对椎间盘退变疾病无特效治疗,组织工程技术的发展为它提供了新的治疗思路。目的:综述了去细胞基质支架构建组织工程椎间盘的研究的研究进展。方法:应用计算机检索PubMed、中国知网、万方、中国生物医学文献库数据库2005至2014年文献,检索关键词为"椎间盘退变,去细胞基质,组织工程,支架,髓核,纤维环;Intervertebral disc degeneration,Extracellular matrix,Tissue Engineering,Scaffold,nucleus pulposus,annulus fibrosus"。结果与结论:组织工程椎间盘构建包括3要素:细胞支架、种子细胞及细胞因子,支架是其中的关键。去细胞基质是其中一类重要支架,近年来成为组织工程椎间盘支架的研究热点,可分为去细胞纤维环支架、去细胞髓核支架及一体化椎间盘支架,其中不同支架具有多种制备方法。相对各种人工材料来源及单一天然蛋白成分来源的支架,去细胞基质具有自己显著的优点,抗原性小、生物相容性高、最为接近正常髓核三维结构,可提供细胞生长所需微环境。但去细胞基质做为组织工程椎间盘支架同时仍存在少量不足,亟待进一步改进。
BACKGROUND：At present there is no specific therapy for the treatment of degenerateive disc diseases. The development of tissue engineering technology provides a new therapy idea for it. OBJECTIVE：To review the research progress of acelular matrix scaffold to construct tissue-engineered intervertebral disc. METHODS：A computer-based online search of PubMed, CNKI, Wanfang and Chinese Biomedical Database was performed for relevant articles published between 2005 to 2014 using the keywords of “intervertebral disc degeneration, extracelular matrix, tissue engineering, scaffold, nucleus pulposus, annulus fibrosus” in English and Chinese, respectively. RESULTS AND CONCLUSION：Construction of tissue-engineered intervertebral disc includes three essential elements： cel scaffolds, seed cels and cytokines. Scaffolds are the key. Acelular matrix scaffold is an important scaffold which has been a hot area of research in scaffolds for tissue-engineered intervertebral disc for several years, which can be divided into three types： acelular annulus fibrosus scaffold, acelular nucleus pulposus scaffold and integrated intervertebral disc scaffold. Different scaffolds have a variety of preparation methods. Acelular matrix scaffold has its own significant advantages, such as low antigenicity, good histocompatibility, and a most similarity with the three-dimensional structure of normal nucleus pulposus that can provide a proper microenvironment for cel growth. However, there are stil some disadvantages of acelular matrix scaffold in tissue-engineered intervertebral disc. So, it needs to be further improved.
出处
《中国组织工程研究》
CAS
北大核心
2015年第8期1289-1294,共6页
Chinese Journal of Tissue Engineering Research
