摘要
目的检测结肠癌细胞系中mi R-200a对高转移细胞系Lovo的影响。方法采用荧光定量PCR法检测6种结肠癌细胞系HCT116、HT29,LS174T、SW480、SW620和Lovo中mi R-200a的表达水平,在高转移细胞系Lovo中瞬时转染mi R-200a mimics使其高表达mi R-200a,检测高表达mi R-200a后Lovo细胞增殖、迁移、细胞同质黏附能力和凋亡等情况的变化。结果 mi R-200a在6种细胞系中存在差异性表达,其中具有高转移潜能的Lovo中表达最低,成瘤但不转移的细胞系HCT116表达最高。在Lovo细胞高表达mi R-200a后,Lovo细胞的增殖和迁移能力明显减弱、细胞同质黏附能力增强且凋亡增加。结论 mi R-200a在结肠癌细胞系中表达失常,其作用可能与结肠癌细胞的侵袭和转移有关,高表达mi R-200a能抑制Lovo细胞增殖和迁移、增强细胞间黏附能力、促进凋亡,其分子机制有待进一步研究。
Objective To detect mi R- 200 a expression in human colorectal carcinnoma（CRC） cell lines and explore the role of mi R-200 a in regulating the biological behavior of CRC cells. Methods Real-time quantitative RT-PCR（q RT-PCR） was used to detect mi R- 200 a expression levels in 6 CRC cell lines（HCT116, HT29, LS174 T, SW480, SW620 and Lo Vo）. mi R- 200 a mimics were transiently transfected into Lo Vo, and the changes in cell proliferation, apoptosis, migration, and cell-cell adhesion were assessed using CCK- 8 assay, TUNEL assay, transwell migration assay, and homogenous adhesion experiment, respectively.Results The expression of mi R-200 a was down-regulated in the 6 CRC cell lines, among which the highly metastatic Lo Vo cell line showed the lowest expression and the tumorigenic but non-metastatic CRC cell line HCT116 had the highest expression.Overexpression of mi R- 200 a depressed cell proliferation and migration but promoted cell apoptosis and cell- cell adhesion in Lo Vo cells. Conclusion mi R-200 a plays a role in regulating the invasiveness and metastasis of CRC, and overexpression of mi R-200 a causes a significant reduction of cell proliferation and migration and promotes apoptosis and cell-cell adhesion in Lo Vo cells.
出处
《南方医科大学学报》
CAS
CSCD
北大核心
2015年第3期450-454,共5页
Journal of Southern Medical University
关键词
miR-200a
结肠癌
增殖
迁移
凋亡
粘附
miR-2000a
colorectal carcinnoma
proliferation
migration
apoptosis
adhesion
