摘要
对基质育苗后水培的菘蓝进行缺氮与复氮处理,分析其生长情况及氮代谢产物含量的变化,探讨缺氮和复氮对菘蓝幼苗生长及氮代谢的影响,以提高菘蓝产量和品质以及栽培过程中的氮素利用效率。结果显示:(1)正常供氮条件下,菘蓝幼苗的叶绿素含量、谷氨酰胺合成酶(GS)活性、硝态氮含量、靛玉红含量为最高,而其株高、主根直径、根的鲜重与干重、叶的鲜重与干重、根系活力均最小。(2)缺氮处理增加了菘蓝幼苗的主根直径和根干重,提高其根系活力和硝酸还原酶(NR)活性,促进游离氨基酸在叶中的积累;但降低了GS的活性,也降低了叶中硝态氮、可溶性蛋白、靛玉红及根中游离氨基酸的含量;缺氮对叶中靛蓝的含量无明显影响。(3)复氮处理增加了菘蓝幼苗的株高、主根长、根鲜重、叶鲜重、叶干重,提高了其根系活力,降低了NR和GS的活性;与对照相比,复氮降低了叶中硝态氮含量,提高了叶中可溶性蛋白、靛蓝及根中游离氨基酸的含量,但对叶中游离氨基酸和靛玉红含量影响较小。研究表明,缺氮后再复氮有利于菘蓝幼苗叶的生长,同时有利于增加其叶内靛蓝含量,从而提高其产量和品质。
The seedlings of Isatis indigoticain hydroponic cultivation,which seeds were planted in media,were treated by N deficiency and N recovery,and the growth and contents of nitrogen metabolites were analyzed to study the effect of N deficiency and N recovery on growth and nitrogen metabolism of I.indigoticaseedlings,and so as to increase the yields,quality and nitrogen utilization.The results showed that：（1）The content of chlorophyll,nitrate,indirubin,glutamine synthetase（GS）activity were the largest,and plant height,main root diameter,fresh weight and dry weight of root,fresh weight and dry weight of leaf and root activity were minimal under the normal treatment.（2）N deficiency could increase main root diameter and dry weight of root,improve the root activity and the activity of nitrate reductase（NR）,promote the accumulation of free amino acid in the leaves.Meanwhile,it reduced the GS activity,the contents of nitrate,soluble protein and indirubin in leaves and free amino acids in roots.There were almost no influences to the content of indigo in leaves under N deficiency.（3）N recovery enhanced the weight of plant height,root length,root fresh weight,leaf fresh weight,dry weight of leaves,improved its root activity and reduced the NR and GS activity.Compared with normal process,N recovery reduced the contents of nitrate nitrogen inleaves,improved the content of soluble protein,indigo in leaves and free amino acids in roots.There were little influences to the contents of free amino acids and indirubin in leaves.These results indicated that N recovery after N deficiency was beneficial to the leaf growth and the increasing of indigo of I.indigotica seedlings.
出处
《西北植物学报》
CAS
CSCD
北大核心
2015年第3期523-529,共7页
Acta Botanica Boreali-Occidentalia Sinica
基金
国家自然科学基金(31171486)
国家大学生创新创业训练计划(201410307026)
关键词
缺氮
复氮
菘蓝
生长
氮代谢
N deficiency
N recovery
Isatis indigotica Fort.
growth
nitrogen metabolism
