摘要
目的 探讨微小RNA （miR）-487b对胶质瘤细胞增殖和凋亡的影响.方法 通过实时荧光定量PCR（qRT-PCR）检测6例非肿瘤脑组织、6例胶质瘤组织中miR-487b的表达;体外培养人胶质瘤细胞LN229将其分为两组：对照组（转染无义序列作为对照）,实验组（转染人工合成miR-487b模拟体）,qRT-PCR检测转染后细胞中miR-487b的表达水平;采用MTT检测LN229细胞增殖情况;流式细胞术检测LN229细胞周期及凋亡;结果 miR-487b的表达量在胶质瘤组织中（2.67±0.90）× 10-2较非肿瘤脑组织（1.23±0.22）含量低（P＜0.05）,miR-487b模拟体上调了LN229细胞中miR-487b的表达水平（与对照组比较P＜0.05）,MTT结果显示miR-487b能明显抑制LN229细胞的增殖（与对照组比较P ＜0.05）;流式细胞术检测细胞周期发现miR-487b可将LN229细胞阻滞于G0/G1期（与对照组比较P＜0.05）;流式细胞术检测细胞凋亡发现miR-487b组细胞凋亡比例较对照组明显增加（P＜0.05）.结论 miR-487b可有效抑制胶质瘤细胞增殖并促进其凋亡,可成为胶质瘤诊断和治疗的候选靶点.
Objective To investigate the effect of microRNA （miR）-487b on the proliferation and apoptosis of glioma cells by observing the changes of cell proliferation,cell cycle and apoptosis.Methods The expression of miR-487b in 6 samples of glioma tissues and 6 samples of non-tumor control brain tissues was detected by using real-time PCR （qRT-PCR）.After artificially synthesized miR-487b mimics was transiently transfected into LN229 glioma cells,the expression of miRNA-487b was tested by qRT-PCR.MTT assay was applied to detect the cell proliferation.The cell cycle and apoptosis were measured by flow cytometry.Results MiR-487b was down-regulated in glioma samples（2.67 ±0.90） × 10-2 compared with non-tumor samples （1.23 ± 0.22）.The transient transfection of miR-487b mimics into LN229 glioma cells significantly increased the expression of miR-487b （compared with normal control,P 〈 0.05）.The result of MTT assay showed that miR-487b might inhibit LN229 cells proliferation significantly （compared to normal control P 〈 0.05）.The cell cycle analysis detected by flow cytometry assay showed that miR-487b raised the cell proportion in G0/G1 phase （compared to normal control,P 〈0.05）.The apoptosis rate detected by flow cytometry assay showed that miR-487b might promote the cell apoptosis （compared with normal control,P 〈 0.05）.Conclusion MiR-487b might decrease glioma cells proliferation,block cell cycle,and promote cell apoptosis,which indicated that miR-487b was a new target for the diagnosis and treatment of glioma.
出处
《中华神经外科杂志》
CSCD
北大核心
2015年第3期309-312,共4页
Chinese Journal of Neurosurgery
基金
天津市科委抗癌重大专项攻关计划项目（12ZCDZSY17700）
天津市科委重点项目（14JCZDJC35600）
天津市卫生局科技攻关项目（11KG115）
